Enzyme Activity Measurement of Hyaluronoglucosaminidase

Creative Enzymes’ advantages in enzyme activity testing include the exceptional development ability and high service quality. Having testified by a great number of customers, our supreme and rapid services are well recognized in the industry. Backed by the most experienced enzymologists, we are proficient in hydrolases activity assays, especially the measurement for hyaluronoglucosaminidase.

Hyaluronoglucosaminidase (hyaluronidases, EC 3.2.1.35) catalyzes the hydrolysis of hyaluronic acid (HA), a high molecular weight non-sulfated linear glycosaminoglycan, which is composed of repeating disaccharide units, D-glucuronic acid (GlcA) and N-acetyl glucosamine (GlcNAc). The enzyme cleaves the beta-1,4-glycosidic linkage between N-acetyl-glucosamine and glucuronate within the HA chain, producing non-reactive oligomeric end products. They also hydrolyses 1,4-beta-D-glycosidic linkages between N-acetyl-galactosamine or N-acetylgalactosamine sulfate and glucuronic acid in chondroitin, chondroitin 4- and 6-sulfates. To a small extent, they hydrolyze dermatan sulfate. Hyaluronidases are wide spread in nature, being found in mammals, insects, leeches, and bacteria. The diversity allows them to have different catalytic mechanisms, substrate specificities, affinities to inhibitors, and notably, different pH optima.

Hyaluronoglucosaminidases have different roles in different species. In venoms of various organisms, such as those of bees, wasps, spiders, scorpions, fish, snakes and lizards, hyaluronidases enhance the noxious action of substances by helping to spread constituents of the venom cocktail at the injection site. In humans, increased levels of hyaluronidase are found in some cancers, such as colorectal, bladder, prostate, and breast cancer. Therefore, the enzyme is used as a marker for the diagnosis of growing tumors. The enzyme is being heavily studied for its versatile functions. To this end, Creative Enzymes offers accurate assay services for hyaluronoglucosaminidase by spectrophotometric assays. The enzyme activity is measured by the quantification of free reducing ends of hydrolyzed substrate. Considering the instability under some conditions, Creative Enzymes provides more targeted methods to satisfy your specific requirements.

Figure: The crystal structure of bee venom hyaluronidase in complex with hyaluronic acid tetramer.
Reference: Markovic-Housley, Z. et al. Structure. 2000 8(10): 1025-35.

Creative Enzymes uses the advanced instrument to generate the most reliable results in enzyme activity determination. Besides, we have a powerful research team and extensive experiences to make sure of the high-quality services and rapid turnover. We are continuously upgrading the techniques and renewing the expertise in emzymology. Overall, Creative Enzymes is the best choice for enzyme activity measurement.