Enzyme Expression and Purification

Creative Enzymes has decades of experiences in enzyme expression and purification. We provide a series of services including gene cloning, expression, and fermentation optimization and production of enzymes. The crude enzyme product is then purified properly to be stored or used with stable activities over time. On the top of enzyme expression, we also provide the purification service for enzymes from natural sources or customized designs.

Enzymes Expression Service Workflow

Creative Enzymes will best tailor these services based on customers’ research needs. With extensive experiences in enzyme production, we are able to offer the best services:

For enzyme expression and production

	Host	Services	Timeline	Price
Enzyme Expression & Production		Gene identification, synthesis and cloning	2 weeks	From $2,000/mg of enzyme Get a quote
		Codon optimization	1-2 weeks
		Selection and modification of expression system	1 week
		Selection and construction of expression vector	1-2 weeks
		Tag selection	1 week
		Protein refolding	1-2 weeks
		Post-translational modifications	2-3 weeks
		Identification and optimization of enzyme expression conditions	1-2 weeks
		Scale-up enzyme expression	1-2 weeks
		Fermentation development and consulting	2-3 weeks

For enzyme purification

FAQ

Q1: What expression host should I use for my enzyme?

Answer: Using our powerful expression platform, we will select the most suitable host based on the need of specific enzymes, with consideration of purity, biological integrity, and potential toxicity. We provide competent and stable expression systems included bacterial expression systems, yeast expression systems, baculovirus expression systems, and mammalian cell expression systems, which all have unique advantages as listed below.

For special enzymes that have not been expressed in a certain system, we also offer expression system screening services.