Nanozymes for In Vivo Imaging

The growth of novel in vivo imaging modalities has led to an array of new names in the past few years. Imaging with nanozymes instead of conventional methods can afford intelligent control of delivery, and at the same time, greatly increase diagnostic sensitivity and specificity. In other cases, the use of nanozymes actually enables the use of contrast agents for imaging modalities where they are available, making nanomaterials a driver of molecular imaging capabilities for imaging modalities. In even other cases, nanomaterials are simply required to make imaging possible.

Chemists working closely with imaging scientists have played a key role in the development of these novel materials to address some of the critical issues facing diagnostic imaging. Creative Enzymes offers a variety of nanozymes for in vivo imaging, including photoacoustic imaging and nanozymes probes.

Photoacoustic Imaging with Nanozymes

• Creative Enzymes designs a quantum-dot-based nanozyme for in vivo H₂O₂-responsive catalytic photoacoustic imaging.
• Graphene quantum dots show intense peroxidase activity and effectively catalyze the peroxidase substrate 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS) into its oxidized form. The oxidized ABTS then exhibits strong near-infrared (NIR) absorbance, rendering it to be an ideal contrast agent for photoacoustic imaging.

Fig. 1 Schematic illustration of an exosome-like enzyme vesicle for H₂O₂-responsive catalytic photoacoustic imaging. (Ding H, et al., 2019)

• We achieve tumor phototherapy, simultaneous photoacoustic / thermal imaging, and computed tomography by using iridium oxide catalase nanozymes, which have extraordinary photothermal conversion efficiency and X-ray absorption coefficient.
• This thus opens novel avenues to nanomedical applications in which nanozymes can lead to a better understanding of nanomedicine uptake, biodistribution, and pharmacokinetics.

Nanozymes Probes for Imaging

• We prepare a tumor-microenvironment-activated nanozyme-mediated theragnostic nanoreactor for imaging-guided therapy.
• The constructed activatable nanoreactors achieve non-invasive imaging of tumor progression, using nanozyme-mediated photoacoustic imaging signal and photothermal therapy (PTT) function.
• We also provide a mesoporous carbon-gold hybrid nanozyme probe for real-time imaging, photothermal / photodynamic and nanozyme oxidative therapy.
• The results demonstrate that the synthesized nanozyme probes reveal excellent tumor targeting efficacy, long tumor retention, and favorably diagnostic and therapeutic effect for tumor.

Various Disease Imaging Based on Nanozymes

• Nanozymes also have been broadly exploited for many other diseases imaging such as tumors, infections, inflammation and some neurological diseases.
• We develop gold nanozymes, which can penetrate and accumulate inside the acidic microenvironment of biofilms. Therefore, they enable imaging detection of biofilm-associated infections arising from different and/or hybrid bacterial species.
• We provide a nanozyme tag, which enables chemiluminescence imaging probe for simultaneous multiplex imaging of cytokines.

Creative Enzymes provides customized nanozymes for in vivo imaging. We offer a broad range of customized R&D services and deliver solutions tailored to our customers' unique specifications. If you are interested in our services or have some questions, please feel free to contact us or make an online inquiry.