Enzyme Extraction and Isolation

Enzymes are a class of proteins with catalytic effects that are involved in important processes of biological activities. With the research and development of science and technology, more and more techniques are applied in the field of enzyme research. The extraction and isolation of enzymes are of great significance in the field of enzyme synthesis and therefore have attracted more and more attention from scientists all over the world.

Creative Enzymes relies on its rich industry experience, professional knowledge and technology platform to provide customers with comprehensive solutions about enzyme extraction and isolation.In enzyme extraction and isolation, we will extract the enzyme from the tissue and subsequently isolate and purify it. Generally, we will choose the appropriate method to extract and isolate enzymes of different purities according to different research purposes and work requirements. In addition, we also measure the activity of the extracted and separated enzymes to maximize the quality of the enzymes we provide.

Significance of Enzyme Extraction and Isolation

As biological catalysts of life, enzymes play an indispensable role in various biological processes. From food production to pharmaceuticals, enzymes have important applications in many industries, and the extraction and isolation of high-purity, high-vitality enzymes play an important role in enzyme synthesis. In our many years of enzyme research, we aim to provide effective, comprehensive solutions to the complexities of enzyme extraction and isolation.

What do we Offer?

Enzyme Extraction. It is the process of treating the enzyme-containing raw material after cell fragmentation with a suitable solvent under certain conditions so that the enzyme is fully solubilized into the extraction solution. Extraction methods use various techniques to extract enzymes directly from the cells or tissues of plants, animals, or microorganisms. Currently, we offer the following enzyme extraction methods.

• Overcolumn method.
• Salt solution extraction method.
• Alkali solution extraction method.
• Organic solvent extraction.

Separation of enzymes. We target to isolate a variety of enzymes contained in the extract, and to isolate and purify a particular enzyme from the extract, we will choose a suitable isolation and purification method based on the characteristics of this enzyme. There are many methods for enzyme separation and purification, and we will choose different separation methods according to the specific research objectives. Some of the methods utilized in enzyme separation and purification are as follows.

• Gel Electrophoresis. We use the colloid electrophoresis method to purify large molecules of biological enzymes, this method in the separation and purification of enzymes with a simple operation, separation effect is good.
• Ion Exchange Separation Method. The ion exchange separation method is a method of separating proteins from other proteins by utilizing the electrostatic adsorption property of ion exchange resin. We use this method in cases where the number of proteins in the bio-enzyme extract is relatively small.
• Column Chromatography. Column chromatography is a method in which the enzyme solution is passed through a column equipped with a layered column, and the proteins in the mixture are separated and purified by various column chromatography methods. We generally utilize this method when separating small molecules of biological enzymes.
• Aggregation Method. We utilize this method to enable large-scale separation and purification of enzymes from the environment.

Our Experimental Process

Creative Enzymes is dedicated to the field of enzyme research and continues to provide comprehensive and effective solutions for the synthesis of high quality enzymes. Our enzyme extraction and isolation solutions play an important role in helping our customers to obtain their target enzymes and advance the field of enzyme synthesis. If you are interested in us, please feel free to contact us.