Virus Detection with Nanozymes

Viruses have a fairly simple structure, usually consisting of a protein capsid and a nucleic acid core. Common virus detection methods include pathogen culture, nucleic acid detection, immunological methods and so on. Pathogen culture and nucleic acid detection require special experimental equipment and professional technical personnel, and the technology is complicated. Immunological methods include enzyme-linked immunosorbent assay (ELISA), immunofluorescence, colloidal gold immunochromatography, etc.

Creative Enzymes develops an advanced platform to provide simple, fast and accurate detection of viruses based on nanozymes with decades of experience. Our experts will answer your questions comprehensively and carefully and help you solve the problems in your studies. We guarantee satisfied and reliable results for our customers all over the world.

Anti-Influenza Virus Therapy with Nanozymes

• Some nanozymes possess magnetic properties, which can be used to isolate and enrich viral biomarkers by applying external magnetic field to further improve the specificity of detection.
• Creative Enzymes proposes a magnetic Mag NBs-based nanozyme-linked immunosorbent assay (Mag LISA) by combining Fe₃O₄ magnetic nanobeads and Au nanozymes, which can not only be used for magnetic separation of influenza A virus molecules, but also for signal amplification through TMB color development catalyzed by peroxidase-like activity. Thus, one-step identification, isolation and visual analysis of influenza virus can be achieved.

Detection of Respiratory Syncytial Virus with Nanozymes

• Respiratory syncytial virus is a single stranded RNA virus of paramyxovirus genus, which can cause respiratory diseases. Due to the lack of safe and effective vaccines, the development of highly sensitive virus detection methods for early diagnosis and treatment is particularly important.

Fig. 1 Schematic representation of the Hg²⁺ -Au-GO hybrids for colorimetric immunoassay of respiratory syncytial virus.

• We develop a colorimetric immunoassay based on the hybrid of mercury ion-gold nanoparticles and GO, the hybrid nanozymes exhibit significant peroxidase activity. In the presence of H₂O₂, TMB can be oxidized to produce a color reaction.

Detection of Novel Coronavirus with Nanozymes

• Chemiluminescence immunoassay is also a common detection method, which combines the high sensitivity of Chemiluminescence with the good specificity of immunoassay.
• Luminol is a common chemiluminescence reagent. The enzyme catalyzes the oxidation of luminol by H₂O₂ to produce blue light.

Fig. 2 Nanozymes chemiluminescence test strip for detection of SARS-CoV-2S-RBD.

• We construct a chemiluminescence test strip of nanozyme by binding cobalt-Fe@hemin nanozyme to specific antibody of novel coronavirus antigen SARS-CoV-2S-RBD. Luminol substrate catalyzes chemiluminescence signal in the presence of H₂O₂. The chemiluminescence signal is captured and analyzed, which provides an economical and simple screening method novel coronavirus (COVID-19).

Creative Enzymes offers virus detection based on nanozymes with customized delivery strategies and precise design. Our advanced technical platforms help our clients solve the problems they may encounter in research based on decades of experience. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.