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High-throughput Enzyme Screening
About High-throughput Enzyme Screening
High-throughput enzyme screening is a method for rapidly screening large numbers of enzyme samples using automation and parallel processing techniques. Traditional screening methods (e.g., agar plate and microtiter plate screening) do not allow for high-throughput and rapid screening. With the improvement of instrumentation and the rapid development of biotechnology, many techniques with ultra-high-throughput screening capability have dramatically increased the screening throughput, such as fluorescence-activated cell sorting (FACS) based on flow cytometry and droplet-based microfluidic sorting (DMFS) based on microfluidic chip and sorting device. They allow simultaneous testing of multiple samples to assess key properties such as enzyme activity, substrate specificity, and stability in a short period of time. High-throughput enzyme screening accelerates the process of enzymology research, enzyme engineering, and drug discovery with improved efficiency and accuracy.
Our High-throughput Enzyme Screening Services
Creative Enzymes provides professional high-throughput enzyme screening services and is committed to providing our clients with fast, accurate, and reliable enzyme screening solutions. With advanced instrumentation and a team of experienced scientists, we are able to handle large numbers of samples and provide high-quality data analysis and interpretation.
Our High-Throughput Enzyme Screening Service Technology
Fluorescence-activated cell sorting (FACS)
We offer FACS-based high-throughput enzyme screening services. FACS is a more maturely developed technology, which is more suitable for the screening of target products in cells or on cell membranes. According to the different forms of fluorescent products coupled with enzymes and their encoding genes, the existing FACS enzyme activity screening systems can be categorized into cell membrane surface display, enrichment of intracellular fluorescent products, and fluorescent protein expression activity report.
Droplet microfluidic sorting (DMFS)
We offer DMFS-based high-throughput enzyme screening services. The DMFS screening method is not only applicable to the screening of intracellular enzymes but also to the screening of extracellular secreted enzymes.
High-throughput microtiter plate
We also use high-throughput microtiter plate technology. High-throughput microtiter plates contain multiple wells, each of which can hold one enzyme sample. By performing reactions and measurements on a microtiter plate, multiple enzyme samples can be tested simultaneously and high throughput enzyme activity and substrate conversion data can be obtained.
In addition to the above high-throughput enzyme screening techniques, we can also use other parallel processing techniques and tools, such as microfluidic chips and nanopore arrays, to improve the screening efficiency and sample processing capacity.
Our High-throughput Enzyme Screening Services Process
- Consultation and project planning: Customers are required to provide information about the target enzyme, such as enzyme type, source, and properties. We will design the most suitable screening program according to the customer's needs and enzyme properties, including the selection of appropriate enzyme substrates, optimization of screening conditions, and so on.
- Sample preparation: You can provide the enzyme samples, or we can perform enzyme expression, purification, and activity assay according to your requirements to ensure the high quality of the samples and suitable preparation for high-throughput screening.
- High-throughput screening experiments: We utilize high-throughput platforms and automated instruments to perform enzyme library screening and activity analysis experiments.
- Data analysis and reporting: Our team will perform comprehensive data analysis of the screening results and generate detailed reports. The report will include data related to the experimental results, as well as interpretations and recommendations of the screening results.
- Enzyme sample provision: Upon request, we can provide high-purity enzyme samples for your further enzyme research and applications. Our enzyme samples have a long shelf life to ensure their stability and reliability.
Our high-throughput enzyme screening service can help customers obtain the required target enzymes quickly and efficiently, and provide relevant data and enzyme samples to provide strong support for your research or application. Please contact us for more details about our high-throughput enzyme screening services and let us customize the most suitable solution for you.