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Cellular life fundamentally depends on a continuing network of molecular interactions among proteins, nucleic acids, and small molecules. Aberrant interactions are usually the cause of most human diseases such as cancer or autoimmunity. Since proteins rarely act alone as their functions tend to be regulated, the study of protein interactions with different biological compounds, such as enzymes, DNA, RNA, and other small molecules, empowers the current knowledge on biochemical cascades and molecular etiology of disease, as well as the discovery of putative protein targets. In enzyme discovery, the study of protein interactions can also contribute to the development of screening methods.
The complete map of protein interactions that occur in an organism is called the interactome, which could only be achieved by a collective effort between the experiments and computations. The most popular experimental methods for protein interactions are the yeast-two-hybrid (Y2H) system, affinity purification followed by mass spectrometry (AP-MS), and literature-derived low-throughput experiments. These approaches are capable of determining the types and characteristics of protein interactions, which contributes to various databases of known protein interfaces, such as ProtCID and 3DID. Limitations of experimental methods necessitate computational prediction of protein interactions. The majority of existing various computational approaches can be grouped as simulation-based and statistical/machine-learning-based approaches. The protein interaction prediction methods include interior search, gene cluster and gene neighborhood, residue coupling, docking, and gene coexpression profiles.
To fulfill the need for efficient enzymes in different industries, Creative Enzymes is dedicated to protein interaction-based enzyme screening. Our protein interaction-based enzyme screening service utilizes information on protein interactions to help clients discover and optimize enzymes with specific functions. By studying enzyme interactions with substrates, ligands, or other proteins, we are able to determine the active site, substrate specificity, and catalytic mechanism of the enzyme, and provide important guidance for enzyme optimization and application. We utilize our expertise and state-of-the-art equipment to provide comprehensive solutions to meet your specific research needs with the help of advanced experimental strategies and data analysis tools.
Depending on the purpose, we can provide various types of protein interaction methods including in vivo, in vitro, and in silico methods:
Our protein interaction-based enzyme screening services include, but are not limited to, the following:
At Creative Enzymes, we have the advanced technology and experience to provide our clients with high-quality protein interaction-based enzyme screening services. If you are interested in our services or have any questions, please feel free to contact our customer service team. We look forward to working with you and supporting the success of your project!
Figure 1 Conceptual view on protein-protein interaction prediction methods.
(Chemical Reviews 2016)
