About Enzyme Screening Based on Protein Interactions

Cellular life fundamentally depends on a continuing network of molecular interactions among proteins, nucleic acids, and small molecules. Aberrant interactions are usually the cause of most human diseases such as cancer or autoimmunity. Since proteins rarely act alone as their functions tend to be regulated, the study of protein interactions with different biological compounds, such as enzymes, DNA, RNA, and other small molecules, empowers the current knowledge on biochemical cascades and molecular etiology of disease, as well as the discovery of putative protein targets. In enzyme discovery, the study of protein interactions can also contribute to the development of screening methods.

The complete map of protein interactions that occur in an organism is called the interactome, which could only be achieved by a collective effort between the experiments and computations. The most popular experimental methods for protein interactions are the yeast-two-hybrid (Y2H) system, affinity purification followed by mass spectrometry (AP-MS), and literature-derived low-throughput experiments. These approaches are capable of determining the types and characteristics of protein interactions, which contributes to various databases of known protein interfaces, such as ProtCID and 3DID. Limitations of experimental methods necessitate computational prediction of protein interactions. The majority of existing various computational approaches can be grouped as simulation-based and statistical/machine-learning-based approaches. The protein interaction prediction methods include interior search, gene cluster and gene neighborhood, residue coupling, docking, and gene coexpression profiles.

Depending on the purpose, we can provide various types of protein interaction methods including in vivo, in vitro, and in silico methods:

• Experimental methods for protein interaction analysis, including co-immunoprecipitation (co-IP), pull-down assay, crosslinking protein interaction analysis, label transfer protein interaction analysis, and far-western blot analysis.
• Computational methods for protein interaction analysis.
• Proteome-scale interactome studies.

Our protein interaction-based enzyme screening services include, but are not limited to, the following:

• Target protein selection and interaction analysis
• Substrate and ligand screening
• Enzyme activity and characterization
• Enzyme optimization and modification

Why Choose Our Service?

• Rich experience: We have a multidisciplinary team of professionals in enzymology, structural biology, computational chemistry, and biochemistry with rich experience and expertise in the field of enzyme technology.
• Customized service: We can provide tailor-made enzyme screening solutions according to customer needs. We will work with you to understand your project objectives, time, and budget requirements, and design the optimal screening program according to your requirements.
• Multi-disciplinary applications: Our enzyme screening services are applicable to a wide range of fields, including pharmaceuticals, agriculture, and biotechnology. Whether you need to develop new drugs, optimize production processes, or improve the performance of existing enzymes, we can provide you with effective solutions

At Creative Enzymes, we have the advanced technology and experience to provide our clients with high-quality protein interaction-based enzyme screening services. If you are interested in our services or have any questions, please feel free to contact our customer service team. We look forward to working with you and supporting the success of your project!

Figure 1 Conceptual view on protein-protein interaction prediction methods.
(Chemical Reviews 2016)