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Enzymes, as a large group of protein-based biocatalysts, are capable of selective catalysis with high efficiency under mild conditions such as ambient temperature and pressure and near-neutral pH. The need for enzyme biosynthesis has become more and more obvious with the deepening understanding of enzymes and the practical needs of social development. The study of enzyme biosynthesis in organisms has attracted a lot of attention from researchers all over the world.
Creative Enzymes relies on its rich industry experience, professional knowledge and technology platform to provide customers with comprehensive solutions about enzyme biosynthesis. We have brought breakthroughs to the field of biology in enzyme biosynthesis by mimicking the way enzymes are produced and work in nature.
We are dedicated to enzyme synthesis research. Relying on biosynthetic methods, we offer various effective technological approaches in enzyme production, optimization of enzyme function, yield, and stability. So far, the enzyme biosynthesis we offer has shown promising applications in biomedical research, industrial applications, and environmental protection applications.
We utilize genetic engineering techniques, e.g., through gene cloning, to insert a gene for a target enzyme, obtained from a natural source or by means of synthetic biology, into the genome of a host organism, e.g., Escherichia coli, Saccharomyces cerevisiae, or other microorganisms, among others, in order to enable its expression. Expression involves the use of suitable promoters and regulatory elements to produce the target enzyme in the host organism. In addition, we utilize gene editing techniques, such as CRISPR-Cas9, to enhance enzyme production capacity by targeted and accurate modification of the genes of the host microorganism.
The application of genetic engineering techniques plays an important role in enzyme biosynthesis. For instance, when employing microorganisms to make enzymes, we can use genetic engineering to change the regulatory genes of the cells. This can change the strains from inducible to constitutive and deterrent to de-deterrent, improving the ability of the bacteria to create enzymes. Additionally, by raising the copy number of structural genes, we can boost the synthesis of cell-specific enzymes. Additionally, we can raise the copy number of structure genes and transfer the structural genes to the recipient medium to boost the production of cellular enzymes, both of which will increase the production of cell-specific enzymes.
Without prior knowledge of the spatial structure and catalytic mechanism of the biocatalysts, directed evolution of enzymes can increase the efficiency of gene evolution by introducing mutations into the genes that edit biocatalysts through various mutation approaches. These altered genes can result in biocatalysts that work better. Then, to find mutant strains with the desired traits in bigger libraries, screening or selection techniques are used. We utilize evolutionary experiments that are effective in altering enzyme properties through continuous mutation and screening.
Creative Enzymes has extensive experience in enzyme biosynthesis. With the constant development and integration of enzyme synthesizing techniques, we play a significant role in assisting researchers in producing effective, highly active enzyme preparations.
