Tumor Immunotherapy with Nanozymes

Tumor immunotherapy is beneficial over traditional therapies because it generates immune memory that can prevent tumor recurrence. However, the immunosuppressive tumor immune microenvironment (TIME) creates a therapeutic barrier to immunotherapy. Attenuation of oxidative stress and hypoxia can elicit immune responses. In addition, the immunogenic cell death of cancer cells induced by some therapies such as PDT and RT can provoke antitumor immune responses by releasing antigens and proinflammatory cytokines.

The intrinsic catalytic properties allow nanozymes to interact with molecular and cellular components of TIME to alleviate immune suppression. Currently, nanozymes can be used as a standalone therapeutic or synergies with other therapeutic strategies for tumor immunotherapy. Creative Enzymes a variety of nanozymes for tumor immunotherapy.

Cu_2-XTe Nanozymes in Tumor Immunotherapy

• Creative Enzymes designs and synthesizes Cu_2-XTe nanozymes with both glutathione oxidase and peroxidase mimics activities, which can simultaneously consume glutathione and hydrogen peroxide in tumor tissues to produce ·OH and O²⁻.

Fig. 1 Cu_2-xTe nanozyme is used to catalyze inflammation and activate antitumor immunity.

• Through the cascade catalytic reaction of the nanozyme, it can promote the polarization of macrophages from M2 to M1, and effectively produce anti-tumor immunity.
• This method can eliminate the primary tumor, and produce a killing effect on the metastatic tumor by activating the immune response. Therefore, it can inhibit tumor metastasis and recurrence.

IMSN Nanozymes in Tumor Immunotherapy

• We propose an immunomodulation-enhanced tumor catalytic therapy strategy based on iron manganese silicate nanozymes (IMSN), to achieve the synergism between nanozymes and tumor microenvironment (TME) regulation.
• IMSN nanozymes exhibit both intrinsic peroxidase-like and catalase-like activities under acidic TME, which can decompose H₂O₂ into hydroxyl radicals (·OH) and oxygen (O₂), respectively. In addition, IMSN nanozymes regulate the TME, resulting in macrophage polarization from M2 to M1. Thus, they induce the regeneration of H₂O₂, which can promote the catalytic activities of IMSN nanozyme.

GHZD Nanozymes in Tumor Immunotherapy

• We fabricate biomimetic cascade enzyme-initiated toxic-radical-generating devices (GHZD), which are fabricated by various types of nanozymes for enhanced biocatalytic immunotherapy.

Fig. 2 Schematic illustration of in situ C-centered radicals-augmented enzyme-based catalytic anticancer and immune therapy. (Zhao Y, et al., 2021)

• Nanozymes exhibit amplified multienzyme-mimic, such as glucose oxidase, peroxidase, and glutathione peroxidase, cascade reactions in artificial nanoscale proximity.
• Thus, the nanozymes can catalyze the generation of free radicals (·C and ·OH) in a cascade, selectively enhancing the oxidative stress of tumor sites and triggering immunogenic cell death (ICD) of tumor cells. More importantly, they can produce long-term immune effects to inhibit tumor growth and metastasis.

PHCN Nanozymes in Tumor Immunotherapy

We develop a novel PHCN nanozyme that can reshape the tumor microenvironment, enhance the antitumor effect of antigen receptor (CAR) T cells, and improve human immunity.

Creative Enzymes is pleased to share our cutting-edge technology and extensive expertise in nanozymes to facilitate our clients' research and project development. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.