Nanozymes for Detection of Pesticide Residues

Pesticides are used in agriculture to protect crops and seeds before and after harvest. Despite their huge economic benefits to society, pesticide residues in food ingredients can have harmful effects on human health. In addition, the widespread use of pesticides also raises serious concerns about food safety, as pesticide residues are easily exposed to primary and derived agricultural products.

To achieve robust detection of pesticides, we develop and highlight several analysis techniques based on nanozymes. Creative Enzymes provides pesticide detection with nanozymes, helping our customers worldwide in the field of nanozymes. Our platform is equipped with cutting-edge facilities and professional experts to support research. Here, we provide various services according to the requirements of our customers.

Analysis of Organophosphates Pesticides with Nanozyme

• Among the toxic pesticides, organophosphorus pesticides (OPPs) have been reported as the major contaminants in the water, fruit, or medicinal plants.
• Creative Enzymes uses Fe₃O₄ as a peroxidase mimetic enzyme to catalyze luminol chemiluminescence reaction. In the presence of ethanol, the superoxide anion is removed and the chemiluminescent reaction is significantly suppressed. Therefore, we have developed a detection method for OPPs by competitive binding of phosphate-inactivated phosphorus and ethanol with Fe₃O₄ nanozymes.

Fig. 1 Representative illustration of the nanozyme-based method for the detection of various pesticide residues in food samples.

• We synthesize GeO₂ nanozymes with peroxisome-like activity, which catalyze TMB color in the presence of H₂O₂, and further combine it with enzyme inhibition method to construct a detection system for organophosphorus pesticides.
• We also use modified Pt nanozymes as triazophos-phorus competitors, the peroxisome-like activity of Pt nanozymes catalyze the oxidation of TMB by H₂O₂ to generate the blue oxidation product TMB²⁺ with strong Raman signal, which enables the colorimetric and surface Raman enhanced spectroscopic determination of triazophos-phorus in water and pears.

Analysis of Neonicotinoid Pesticideswith Nanozyme

• Neonicotinoid pesticides are a relatively new class of active ingredients with broad internal absorption, low toxicity, and high insecticidal efficiency against mammals.
• We report a colorimetric assay for the rapid detection of pyrimidine with pyrimidine specific aptamers and Au nanozymes.
• A fluorometry for the selective detection of acetamidine was also developed based on aptamers targeting acetamidine, multiple complementary chains, and gold nanozymes.

Analysis of Triazine and Other Pesticides with Nanozyme

• Once in crops, triazines cause long-term negative effects due to their persistence. Another problem is their easy distribution to other parts of the environment, particularly from soil into groundwater.
• We prepare Fe₃O₄-TiO₂ / rGO nanozymes with hydrogen peroxide activity and photocatalytic efficiency, which can sense of atrazine using TMB as substrate molecules. We have also developed Pd / Pt nanozymes as enzyme markers to detect atrazine.
• With the help of heteroatomically doped graphene nanozymes, we also fabricate a colorimetric nanozyme sensor array for the detection of aromatic pesticides by the TMB / H₂O₂ system.

Creative Enzymes offers professional services covering inflammation treatments with enzymes to meet your research needs. With years of experience in nano-zymes, our advanced platform can help our clients solve types of difficulties and conduct research. If you are interested in our services or have some questions, please feel free to contact us or make an online inquiry.