About Thin Layer Chromatography (TLC)-based Enzyme Screening

Thin-layer chromatography (TLC) is a very important experimental technique for the rapid separation and qualitative analysis of small amounts of substances and is also used to follow the course of reactions. It utilizes the different adsorption capacities of the adsorbent (silica gel) to each component in the sample, i.e., the different desorption capacities of the spreading agent to them, so that the components can be separated. In the field of enzyme screening, TLC is widely used to evaluate properties such as enzyme activity, selectivity, and stability. The technique is not only simple, fast, and economical, but also provides efficient enzyme screening services.

Our TLC-based Enzyme Screening Services Process

1. Consultation and project planning: Our professional team will communicate with you to understand your TLC-based enzyme screening needs and goals. We will discuss in detail key factors such as experimental design, substrate selection, and reaction conditions, and develop a strategy that fits your project.
2. Sample preparation: Depending on customer needs, the company can provide sample preparation services, including purification, concentration, and labeling of compounds.
3. Thin-layer chromatography condition optimization: Our technicians fully understand the characteristics of different enzymes and can design the best experimental conditions according to customer needs, including carrier selection for gas chromatography, sample application methods, unfolding agents and detection methods, etc., and accurately control the reaction conditions.
4. Enzyme screening (separation and detection): We design and execute TLC enzyme screening experiments according to customers' requirements, which can be realized by visible light, fluorescence staining, or other detection methods. This helps customers to evaluate enzyme activity, selectivity, and stability and select the best enzyme candidate.
5. Data analysis and reporting: Our team will perform a comprehensive data analysis of the experimental results and generate a detailed experimental report. The report will include a quantitative analysis of enzyme activity, an assessment of substrate specificity and selectivity, as well as interpretation and recommendations of the screening results. Clients can make proper decisions and further research plans based on these reports.

Why choose our TLC-based Enzyme Screening Service?

• Experienced team
• Advanced equipment and technology
• Customized solutions
• Accurate data analysis

Whether you are looking for new enzyme-catalyzed reactions, improving the performance of existing enzymes, or developing efficient, industrially applicable enzyme-catalyzed processes, our TLC-based enzyme screening services can provide you with reliable solutions. Contact our team to learn more about our services and how we can work together.