Preparation of Virus Vaccine with Nanozymes

Vaccination is an important way to effectively prevent virus infection and control the spread of infectious diseases. Conventional vaccines often have the problems of short half-life, weak immunogenicity, weak targeting, slow absorption, high storage and delivery requirements. In recent years, nanozymes are expected to be used in the prevention and treatment of viral infectious diseases due to their lower systemic toxicity, stronger tissue targeting, higher specific surface area and lower immune titer.

Powered by our professional scientists and their years of field experience, Creative Enzymes is able to perform highly accurate and reliable vaccine preparation. With our cutting-edge platforms, we can assess various viruses such as influenza virus, human immunodeficiency virus and so on in a fast and high-throughput manner.

Vaccine of Influenza Virus with Nanozymes

• Au nanozymes always have high surface energy and can spontaneously adsorb viral protein markers, which makes it a good carrier for preparing viral vaccines.
• Creative Enzymes develops a novel influenza A virus vaccine by conjugating the conserved extracellular domain of matrix protein 2 (M2e) of influenza virus to Au nanozymes. As a result, complete protection is achieved after vaccination.

Fig. 1 The action mechanism of nanozymes in antiviral vaccine.

• As a regulator of ROS, nanozymes provide a new idea for the development of catalytic adjuvants for novel viral vaccines.
• We construct a nasal mucosal delivery vaccine with inactivated influenza virus H1N1 using chitosan-modified iron oxide nanozymes as a catalytic adjuvant.
• After intranasal administration, antigen uptake by submucosal dendritic cells, IONzyme can exert POD activity in lysosomes to regulate intracellular ROS levels and promote dendritic cell maturation. In the end, it can achieve the protective effect of H1N1 influenza virus infection.

Vaccine of Human Immunodeficiency Virus with Nanozymes

• Functionalized metal nanozymes, such as gold, silver, and platinum, could be nano adjuvants for HIV vaccine delivery.
• Creative Enzymes uses oligomeric mannosidic-coated gold nanozymes to develop a vaccine that interferes with the GP120-mediated entry of HIV into cells. The nanozymes can inhibit the binding of the 2G12 antibody to gp120 and prevent the 2G12 antibody-mediated neutralization of HIV.
• In addition, negatively charged gold glycosylated nanozymes can stabilize the α-helices and β-lamellae structures of V3 peptides on gp120. The V3-loaded nano vaccine induced antibodies that recognized the GP120 recombinant protein in vivo.

Other ideas for viral vaccines with Nanozymes

• Some studies have shown that some nanozymes, such as TiO₂, ZnO, magnetic iron oxide, can induce conformational changes of proteins, thereby affecting protein function.
• We use nanozymes to interact with key viral proteins and interfere with their normal folding, so that they cannot bind to target molecules, which is a new strategy for designing viral vaccines.

Creative Enzymes offers all the services involved in vaccine preparation based on nanozymes for the convenience of your research. Our goal is to help our clients achieve meaningful results through powerful and consistent approaches. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.