Enzyme Engineering by Site-directed Mutagenesis

Creative Enzymes is the pioneer in several enzyme engineering methods and serves for different research needs. Our scientists are specialized in rational design, accurate computational analysis, efficient experimental operations, and results demonstration. With the technique to change virtually any given base in a cloned DNA, Creative Enzymes builds up a reliable enzyme engineering service through site-directed mutagenesis (SDM).

Site-directed Mutagenesis Workflow

FAQ:

Q1: Which method should I choose to perform site-directed mutagenesis?

At Creative Enzymes, you will always enjoy the superior service which stems from the advanced and latest techniques and reliable experimental materials. Our services cover the two major methods of site-directed mutagenesis.

• For single site-directed mutagenesis, including point mutation, insertion, deletion, and multiple nearby substitutions, we outline reliable method with some modifications, which will benefit researchers in terms of cost savings and time savings.
• For mutagenesis of multiple sites, for example hundreds of base pairs apart, we have combined some efficient methods to a best one. This multiple site method offers a simplified design but also circumvents the need for restriction enzymes during subcloning. In addition, all desired mutations can be completed together with great efficiency using our selected system, which directs the assembly of multiple mutagenic PCR fragments together in one isothermal step.

Creative Enzymes accomplishes site-directed mutagenesis service with high efficiency. The extensive experiences guide us to perform excellent services in a rapid turnover. Our service quality is distinguished from others for high reliability and accuracy. You may contact us anytime for technical support.