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Detection of Glutathione with Nanozymes

Glutathione (GSH) is composed of glutamic acid, cysteine, and glycine. It is an important intracellular regulatory metabolic substance involved in the tricarboxylic acid cycle and glucose metabolism. It can activate a variety of enzymes and plays a very important role in human life activities. Abnormal glutathione levels in the body have been linked to Alzheimer's disease, AIDS, cancer, liver damage, cardiovascular disease and other diseases. Therefore, the development of a simple and reliable GSH detection method is of great significance for biomedicine and disease diagnosis and treatment.

Creative Enzymes is a leading provider of comprehensive, high quality services for nanozymes. We provide an advanced and professional platform for the detection of glutathione with names according to customer needs. We hope to partner with you to explore new frontiers in nanozyme and accelerate your discovery.

Target Nanozymes Detection Method Detection Range Detection Limit Application
Glutathione MnCO3 Colormetric method 0-20 μM  - Detection of GSH in human serum samples.
Glutathione Ag-Au Colormetric method 0.5-1.0 μmol/L - Detection of GSH and glutathione transferase (GST) in serum samples.
Glutathione Au-MnO2 Colorimetric and single particle spectra 0.2-100 μmol/L 0. 018 μmol/L Biosensing
Glutathione Au UV-visible spectrophotometry 0.01-0.2 μg/ml 3.0 ng/ml Environmental sciences, analytical chemistry, and life sciences.

Detection of Glutathione with MnCO3 Nanozymes

  • At first, we use potassium permanganate as manganese source, then, manganese carbonate nanoparticles with oxidase-like activity are prepared by a simple one-step hydrothermal method.

Illustration of (a) the synthesis of MnCO3 nanozymes and (b) GSH detection based on the regulation of oxidase-like activity of MnCO3 nanozymes. - Creative EnzymesFig. 1 Illustration of (a) the synthesis of MnCO3 nanozymes and (b) GSH detection based on the regulation of oxidase-like activity of MnCO3 nanozymes.

  • Under acidic conditions, MnCO3 nanozymes can rapidly oxidize colorless TMB to blue oxidation state product (oxTMB). However, GSH could inhibit the catalytic oxidation process of TMB through reduction, and the color of the solution gradually changed from blue to light color with the increase of glutathione concentration.
  • Based on the principle, Creative Enzymes develops a simple and rapid GSH colorimetric method, and it can successfully apply to the determination of GSH content in human serum samples.

Detection of Glutathione with Ag-Au Nanozymes

  • Creative Enzymes prepares spherical Ag-Au nanozymes with peroxidase activity as a catalyst, to detect glutathione as well as glutathione transferase.
  • We develop a simple, rapid, sensitive and specific colorimetric method for the determination of GSH and GST in human serum samples, and satisfactory results have been obtained.

Detection of Glutathione with Au-MnO2 Nanozymes

  • We compare the blue shift of the local surface plasmon formant of Au-MnO2 nanozymes with the logarithm of the analyte concentration, in order to quantify the concentration of GSH.
  • In particular, the excellent performance of this method is mainly due to the very thin manganese dioxide nanosheets. Au-MnO2 probes based on nanozymes are expected to assess intracellular REDOX status at the single cell level as modified MnO2 is etched within the cell by molecular reduction.

Creative Enzymes provides cost-effective, high quality and hassle-free services to our customers worldwide. With years of experience in biological services, our advanced platforms can help our clients solve various difficulties in detecting glutathione with nanozymes. We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services, please contact us for more details or make an online inquiry.

Our Products Cannot Be Used As Medicines Directly For Personal Use.