Plate-based Screening for Enzymes

About Plate-based Screening for Enzymes

Plate-based enzyme screening is a commonly used method for screening and optimizing enzyme molecules with specific enzymatic activities. The need for this screening method lies in the search for new enzyme-catalyzed reactions, the improvement of the performance of existing enzymes, and the development of enzyme-catalyzed processes that are efficient, specific, and can be applied to industrial production.

Services for Plate-based Screening for Enzymes

Creative Enzymes is a leading enzyme supplier offering a wide range of specialized enzyme screening services designed to quickly and efficiently screen enzymes for specific properties and provide customized solutions for a variety of applications.

Screening service programs based on different media types:

Screening service based on selection medium

By adding or reducing a certain component so that the target strain obtains the growth advantage, while the growth of other strains is inhibited, through several screening and isolation of the final target strain.

Screening service based on detection medium

We mainly based on the colorimetric and fluorescence detection of the screening to be carried out in agar plates, which can also be carried out in microtitre plates.

Usually, a certain reagent or chemical is added to the culture medium, so that a certain identifiable change occurs after cultivation, such as a change in the transparency of the medium or a change in the color around the colony, thus distinguishing between different types of microorganisms or different physiological characteristics.

We offer different plate-based screening service programs to meet different research and application purposes:

Substrate screening service

Enzymes with high catalytic activity towards specific substrates are screened by providing diverse substrates on plates. This helps to determine the substrate specificity of the enzyme and optimize its catalytic performance.

Activity screening service

Enzymes with high catalytic activity are screened by measuring their activity on a plate with the addition of appropriate detection reagents.

Mutagen screening service

By constructing libraries of enzyme variants, we screen plates for enzyme variants with improved or new functions. This helps to improve enzyme properties such as catalytic activity, thermal stability, and substrate specificity.

Why Choose Us?

1. Our services are versatile and flexible. Customers can choose the appropriate enzyme screening platform according to their needs, including common agar plates, microtiter plates, and zymography plates.
2. During the enzyme screening process, customers can optimize the sample handling and reaction conditions according to the specific experimental design requirements in order to obtain the best results.
3. Creative Enzymes also provides customers with a range of assays and analytical methods, including enzyme activity assays, inhibitor screening, affinity assays, etc., to comprehensively evaluate enzyme performance and properties.

Fig.1 Overview of screening technologies. (A) Experimental steps from obtaining the gene library as PCR product to the actual screen. Note that cloning and transformation efficiency are often limiting library size, (B) explanation of symbols, (C) Cell growth/survival selection and agar plate screening, (D) microtiter plate screening, (E) cell as micro-reactor, (F) cell surface display, (G) cell-in-droplet, and (H) in vitro compartmentalization. The methods are explained in the main text. Leemhuis H, et al., 2010)