Description
Soybean trypsin inhibitor (SBTI) is a reversible competitive inhibitor of trypsin and other trypsin-like proteases such as chymotrypsin, plasmin and plasma kallikrein. It has a MW of 22 kDa and a pI of 4. 5. We have immobilized SBTI on an agarose resin via coupling to primary amines to create an affinity resin with specificity for a number of serine proteases. SBTI binds free trypsin tighter with increasing pH from 4. 5-8. 0 that allows for a simple gradient to low pH (~2. 5-3. 0) for elution.
Purity
>95% by SDS-PAGE analysis
Stability
12 months from delivery
Buffer
0.1M Tris-HCl; 0.15M NaCl; 0.02% Na Azide; pH 7. 4
Preservative
Sodium Azide
Synonyms
Trypsin Inhibitor; Immobilized trypsin inhibitor
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