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Enzymes are often stored in a condition which is quite different from their optimal active condition. Therefore, sustaining the activity of the enzyme over a long period is of considerate importance. Herein, Creative Enzymes is fully prepared to offer multiple enzyme immobilization techniques to improve the stability of the enzyme and sustain its activity.
# Required for the guaranteed packages but optional for custom services.
For all these different methods, we provide fully customizable immobilization services in consultation with clients. To discuss more service details, please contact us.
Creative Enzymes has received excellent reputation from countless customers in pharmaceutical and chemical industries. We are considered as the best research partner who is ready to solve any challenge in your research with complete customer satisfaction.
Q: Which methods should I use for enzyme immobilization?
A: Covalent coupling methods are employed when there is a strict requirement for the absence of the enzyme in the product. The functional group that participates in the binding of the enzyme usually involves binding via the side chains of lysine, cysteine and aspartic and glutamic acids. The more recently reported technique for immobilization of enzymes as cross-linked enzyme aggregates (CLEAs) diverges quietly from the conventional immobilization methods. This technique is based on multipoint attachment through intermolecular cross linking between enzyme molecules. Successful preparation of CLEAs from a wide range of enzymes is recently being evaluated by many researchers. The entrapment technique is based on the occlusion of an enzyme within a polymeric network that allows the substrate and products to pass through but retains the enzyme. This technique differs from the coupling technique, in that the enzyme is not bound to the matrix or membrane. There are various methods to entrapping enzymes such as gel or fiber entrapping, and micro-encapsulation. The simplest immobilization method is nonspecific adsorption which is mainly based on physical adsorption or ionic binding. In physical adsorption, the enzymes are attached to the matrix through hydrogen bonding, van der Waals forces, or hydrophobic interactions, whereas in ionic bonding the enzymes are bound through salt linkages.
