About Primary and Secondary Screening of Enzymes

Primary and secondary screening of enzymes is a very important part of modern enzyme biotechnology research. Primary screening is mainly to carry out high-throughput screening of enzymes, screening out enzymes with required characteristics, and providing a basis for subsequent research and application. On the other hand, rescreening is to further verify and confirm the enzyme properties based on primary screening to ensure that the screened enzyme can meet the specific research needs. Over the past decades, the primary and secondary screening techniques for enzymes have evolved and many important advances have been made.

Services for Primary and Secondary Screening of Enzymes

Creative Enzymes is a leading provider of a comprehensive one-stop shop for enzyme screening. Our unrivaled services include both primary screening and rescreening to ensure that our clients have access to the most efficient and reliable enzymes for their specific applications. We have built a state-of-the-art enzyme screening platform combining cutting-edge technology and expertise to provide a streamlined and efficient enzyme screening and optimization process.

Primary screening service

Methods: agar plate coating method, microtiter plate suspension method, genetic engineering.

Service content: efficient enzyme activity assay, binding affinity assay, and enzyme stability assessment.

Characteristics: quick and simple.

By applying a high-throughput screening platform, we screened out most of the non-target strains in a large number of samples and tried to retain potential candidate strains. We can help you obtain highly active, stable, and function-specific enzymes and provide high-quality enzyme samples for subsequent studies.

Secondary screening service

Methods: liquid/gas chromatography, spectrophotometry

Service content: enzyme activity, stability, substrate specificity, pH value, suitable temperature range, structural analysis, catalytic efficiency, substrate specificity, role of inhibitors, etc.

Characteristics: slow, precise.

Our expert team leverages extensive enzyme optimization experience to further refine target enzymes for improved performance after primary screening. Enzyme rescreening is performed through a variety of techniques such as directed evolution, rational design, and protein engineering, which mainly includes improving enzyme activity, stability, substrate specificity, pH, temperature range, etc., to accurately understand the catalytic efficiency of the enzyme, substrate specificity and the effect of inhibitors, etc., and to provide strong support for in-depth research. In addition, the structural analysis of the enzyme can further study the catalytic mechanism of the enzyme and the interaction between the enzyme and the substrate, which provides an important reference for enzyme engineering and drug development. This rescreening ensures that the selected enzymes are optimized to meet the specific needs of the customer's application.

Why Choose Us?

1. High efficiency and rapidity: we have an advanced high-throughput screening platform and experimental equipment, which can quickly screen and analyze a large number of enzyme samples and shorten the research cycle.

2. Diversified technologies: we use a variety of technologies for primary and secondary screening of enzymes, including enzyme activity assay, genetic engineering, enzyme kinetic studies structural analysis, etc., to ensure that the screened enzymes have the required characteristics.

3. Customized solutions: we provide customized enzyme screening solutions according to the customer's needs to ensure that the enzymes obtained can meet specific research or application requirements.

Creative Enzymes is committed to providing a comprehensive and efficient one-stop service for primary and secondary screening of enzymes. Combining our quality services and high-level platforms enables researchers to obtain the most suitable enzymes for their unique projects, thus accelerating progress in various fields such as biotechnology, pharmaceuticals, and biofuels. Feel free to contact us for more details about our enzyme primary and rescreening services.