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Cat No. | Product Name | |
NATE-1698 | Modified T7 RNA polymerase | Get a quote |
NATE-0354 | Native Baker's yeast (S. cerevisiae) Inorganic Pyrophosphatase | Get a quote |
NATE-0355 | Inorganic Pyrophosphatase from Escherichia coli, Recombinant | Get a quote |
NATE-1281 | Inorganic Pyrophosphatase from Saccharomyces cerevisiae, Recombinant | Get a quote |
COV-003 | RNase Inhibitor from Mouse, Recombinant | Get a quote |
NATE-1875 | Native Bovine Deoxyribonuclease I | Get a quote |
VCE-001 | Vaccinia Capping Enzyme | Get a quote |
NATE-1636 | Ribonuclease R from E. coli | Get a quote |
The advent of mRNA vaccines has revolutionized the field of immunization, providing a rapid and flexible platform to combat infectious diseases. The success of mRNA vaccines, such as those developed for COVID-19, relies on sophisticated biotechnological processes that allow for the precise synthesis of mRNA molecules. These molecules are encapsulated in lipid nanoparticles and administered into the human body, where they instruct cells to produce viral antigens. This, in turn, stimulates a specific immune response and creates immunological memory, providing protection against the virus. The in vitro synthesis of mRNA is a critical component of this process, involving several key steps and specialized enzymes. Creative Enzymes, a leading enzyme supplier, offers a comprehensive range of high-quality GMP and non-GMP enzymes that are indispensable for the industrial production of mRNA vaccines.
Figure 1. Creative Enzymes offers a full range of enzymes for in vitro mRNA synthesis.
The synthesis of mRNA in vitro involves a multi-step process, each requiring specific enzymes to ensure high yield and quality of the final product. Below are the essential steps, along with the roles of the required enzymes.
1. Plasmid Linearization
The first step in mRNA synthesis involves the linearization of the plasmid DNA template. This is crucial because linear DNA templates are more efficient for in vitro transcription compared to their circular counterparts.
Enzymes Used:
2. In Vitro Transcription
Once the plasmid is linearized, the next step is the transcription of DNA into mRNA. This is achieved using RNA polymerase, which synthesizes RNA from the DNA template.
Enzymes Used:
3. Capping and Polyadenylation
To make the mRNA functional and stable, it undergoes post-transcriptional modifications, such as capping and polyadenylation. The 5' cap and 3' poly(A) tail are essential for mRNA stability and translation efficiency.
Enzymes Used:
Creative Enzymes is committed to providing high-quality enzyme products and services, essential for the successful in vitro synthesis of mRNA. With a comprehensive product line that includes GMP and non-GMP enzymes, Creative Enzymes supports the efficient and reliable production of mRNA vaccines.
The in vitro synthesis of mRNA is a complex process involving multiple steps and specialized enzymes. The enzymes provided by Creative Enzymes are critical for each stage of this process, from plasmid linearization to mRNA capping and polyadenylation. By offering high-quality enzymes and expert support, Creative Enzymes plays a vital role in advancing the production of mRNA vaccines, contributing to global health solutions.