Description
RNase R is an 3'-->5' exoribonuclease closely related to RNase II, which has been shown to be involved in selective mRNA degradation, particularly of non stop mRNAs in bacteria. RNase R has homologues in many other organisms. When a part of another larger protein has a domain that is very similar to RNase R, this is called an RNase R domain.
Applications
• Alternative splicing studies
• Gene expression studies
• Intron cDNA production
• Intronic screening of cDNA libraries
• Isolation of splicing intermediates and lariats
Product Overview
RNase R is provided as a 250 U size (20 U/μl; 1 μg/μl) and is supplied with a 10X RNase R Reaction Buffer.
Enzyme Commission Number
EC 3.1.13.1
Unit Definition
One unit converts 1 μg of poly-r(A) into acid-soluble nucleotides in 10 minutes at 37°C in 20 mM Tris-HCl (pH 8.0), 100 mM KCl and 0.1 mM MgCl2.
Storage
Store only at -20°C in a freezer without a defrost cycle.
Buffer
RNase R is supplied in a 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 0.1% Triton® X-100 and 1 mM dithiothreitol.
Warnings
RNase R requires low (0.1-1.0 mM) magnesium concentrations for activity. Low EDTA concentrations in substrate RNA solutions can negatively affect RNase R activity. Additional MgCl2, up to 1 mM final concentration can be used to compensate for EDTA in the substrate.
Synonyms
RNase R; Ribonuclease
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