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Recombinant mRNA Cap-2'-O-Methyltransferase
Cat No.
COV-010
Description
mRNA Cap 2´ -O-methyltransferase was derived from a recombinant E. coli strain that carries the gene for the vaccinia mRNA Cap 2´-O-Methyltransferase. This enzyme adds a methyl group at the 2´-O position of the first nucleotide adjacent to the cap structure at the 5´ end of the RNA. The enzyme utilizes S-adenosylmethionine (SAM) as a methyl donor to methylate capped RNA (cap-0) resulting in a cap-1 structure.
The Cap 1 structure can increase the translation efficiency, improving the expression of mRNA in transfection and microinjection experiments. This enzyme specifically requires RNA with an m7GpppN cap as substrate. It cannot utilize RNA with pN, ppN, pppN or GpppN at the 5´ end. Capped RNA may be prepared via in vitro transcription using cap analog or through enzymatic capping using the Vaccinia Capping Enzyme.
The Cap 1 structure can increase the translation efficiency, improving the expression of mRNA in transfection and microinjection experiments. This enzyme specifically requires RNA with an m7GpppN cap as substrate. It cannot utilize RNA with pN, ppN, pppN or GpppN at the 5´ end. Capped RNA may be prepared via in vitro transcription using cap analog or through enzymatic capping using the Vaccinia Capping Enzyme.
Source
E. coli
Applications
To improve mRNA expression during microinjection and transfection experiments.
Form
Liquid
Activity
50 U/μL
Unit Definition
One unit is defined as the amount of enzyme required to methylate 10 pmoles of 80 nt capped RNA transcript in 1 hour at 37°C.
Storage
at -20 °C (Avoid repeated freeze-thaw cycles)
Buffer
20 mM Tris-HC (pH 8.0，25°C), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, 50% glycerol.
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