Description
A modified T7 RNA polymerase that orthogonally recognizes a non-natural promoter sequence: 5’-TAATACCGGTCACTATA, where the underlined residues differ from wild-type.
Abbr
Modified T7 RNA polymerase, Recombinant (E. coli)
Enzyme Commission Number
EC 2.7.7.6
Molecular Weight
100.142 kDa
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50 ul in 1 hour at 37°C in 1X RNA Polymerase Reaction Buffer.
Buffer
50 mM Tris, 50 mM NaCl, 1 mM DTT, 0.1% Triton-X 100, 0.1 mM EDTA, 50% glycerol
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