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BsaI
Cat No.
COV-020
Description
Restriction endonuclease Bsa I is a restriction endonuclease BsaI that is expressed by recombinant E. coli and can recognize specific sites. Restriction endonucleases are widely used in various fields such as gene localization and cloning. This enzyme can quickly cut DNA to achieve efficient gene linearization.
Source
E. coli
Form
Clear colorless solution
Activity
20 U/μL
Purity
≥95%, no DNase, RNase activity, host DNA residue ≤100pg/mg, host protein residue 50ppm, endotoxin residue 10EU/mg, no protease activity, sterile, mycoplasma-free.
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of Internal control DNA in 1 hour at 37°C in a total reaction volume of 50 µL.
Storage
at -20 °C (Avoid repeated freeze-thaw cycles)
Buffer
10 mM Tris-HCl, 200 mM NaCl, 1 mM DTT 0.1 mM EDTA, 200 µg/ml Recombinant Albumin,50% Glycerol. (pH 7.4 @ 25°C).
Warnings
1. The volume of enzyme ≤ 1 / 10 of the reaction volume.
2. Star activity may occur when glycerol concentration is more than 5%.
3. Cleavage activity may occur when Substrate below the recommended ratio.
2. Star activity may occur when glycerol concentration is more than 5%.
3. Cleavage activity may occur when Substrate below the recommended ratio.
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