Description
T7 RNA Polymerase is a DNA-dependent RNA polymerase from T7 phage, with highly specific 5 ´→ 3 ´ RNA polymerase activity, and fewer dsRNA byproducts in the in vitro transcriptional product compared to wild-type T7 RNA polymerase.
Enzyme Commission Number
EC 2.7.7.6
Unit Definition
One unit is defined as the amount of enzyme that will catalyze NTP to produce 1 nmol PPi in 1 hour at 37°C.
Storage
at -25 to -15 °C (Avoid repeated freeze-thaw cycles)
Buffer
50 mM Tris-HCl，100 mM NaCl，20 mM β-ME，1 mM EDTA，50% Glycerol，0.1% (w/v) Triton® X-100，pH 7.9 @ 25°C
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