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Recombinant Deoxyribonuclease I from Bovine, RNase-free
Cat No.
COV-007
Description
DNase I (deoxyribonuclease I, RNase-free) is derived from a recombinant E. colistrain carrying Bovine Pancreatic DNase I. It is an endonuclease that nonspecifically cleaves DNA to release ditri- and oligonucleotide products with 5´ phosphorylated and 3´-hydroxylated ends. This enzyme was originally isolated from bovine pancreas, and the mammalian pancreas is one of the dominating sources until this day. DNase I act on various DNAs such as single and double-stranded DNA, RNA: DNA hybrids, and chromatin (the cleavage rate is affected by histones).
Source
E. coli
Species
Bovine Pancreatic
Applications
Degradation of DNA template in transcription reactions.
Removal of contaminating genomic DNA from RNA samples.
DNase I foot-printing: Used for Foot-printing analysis of DNA-protein interactions.
Nick Translation: Used in conjunction with DNA Polymerase I for nick translation.
Removal of contaminating genomic DNA from RNA samples.
DNase I foot-printing: Used for Foot-printing analysis of DNA-protein interactions.
Nick Translation: Used in conjunction with DNA Polymerase I for nick translation.
Form
Liquid
Enzyme Commission Number
EC 3.1.21.1
CAS No.
9003-98-9
Purity
> 95% (SDS-PAGE)
Unit Definition
One unit is defined as the amount of enzyme which will completely degrade 1 µg of pBR322 DNA in a total reaction volume of 50 µl in 10 minutes at 37°C. Complete degradation is defined as the reduction of the majority of DNA fragments to tetranucleotides or smaller.
Storage
at -20 °C (Avoid repeated freeze-thaw cycles)
Buffer
2 mM CaCl2, 10 mM Tris-HCl (pH 7.6，25°C), 50% glycerol.
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