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Detection of Glucose with Nanozymes
Glucose is the main energy source of most living organisms, and it is also an important metabolite. Likewise, it is indispensable for all living organisms. Detection of glucose plays an important role in biochemistry, clinical medicine (such as the diagnosis of diabetes), and food safety.
Creative Enzymes develops an advanced platform for customers worldwide in the field of glucose detection with nanozymes. We customize featured services according to customer needs with decades of experience in nanozymes. In addition, we deliver satisfactory and reliable results and reports on time to our customers all over the world.
| Target | Nanozymes | Detection Method | Detection Range | Detection Limit | Application |
|---|---|---|---|---|---|
| Glucose | Co3O4 | Spectrum method | - | 10-10 mol/L | Biosensors, immunoassays and medical research |
| Glucose | Pt | Colorimetric method | 1-1000 μM | 0.5 μM | Detection of glucose in human blood |
| Glucose | Cu-MOF | Multi-enzyme simulation system | 0.01 mg/mL-0.3 mg/mL | - | Beverage testing results are good |
| Glucose | MnO2 | Colorimetric method | 0-2 mmol/L | - | One-step detection of glucose |
Detection of Glucose with Co3O4 Nanozymes
- Firstly, we use a simple wet chemical method to synthesize Co3O4 nanozymes, which have both peroxidase-like and glucose-like oxidase activities.
Fig. 1 (a) Nanozyme as peroxidase coupled with glucose oxidase to construct glucose sensor. (b) Nanozyme and oxidase are combined to realize the detection of other target substrates.
- Co3O4 nanozymes can catalyze glucose and O2 to generate gluconic acid and H2O2, and then H2O2 can oxidize TMB to form the blue oxidation product, showing strong SERS signal. Based on this principle, Creative Enzymes develop a novel SERS strategy based on nanozymes to analyze glucose.
Detection of Glucose with Pt Nanozymes
- Creative Enzymes prepares platinum nanozymes by sodium borohydride reduction using linear polyvinyl amine as template. The catalytic activity of TMB is low and its biocompatibility is poor.
- We also provide platinum nanozymes prepared by using ginkgo biloba polysaccharide with good biocompatibility as carrier. Platinum nanozymes significantly blue TMB and detect blood glucose concentrations with approximately 90% accuracy.
- We also develop platinum nanozymes loaded with polygonum and longan polysaccharide, which have almost no cytotoxicity to cells and have weak aggregation in fibrinogen solution for a long time. The accuracy rate of detection of blood glucose concentration by this method is about 95%.
Detection of Glucose with Cu-MOF Nanozymes
- It has been reported that there are abundant catalytic sites on MOF, whose structure and intrinsic properties confer the intrinsic enzymatic activity of MOF. Using MOF as a precursor or template, multiple MOF-based derivatives can be synthesized to construct nanozymes with high stability and catalytic activity.
- Based on the above principle, Creative Enzymes constructs MOF with peroxidase-like activity (Cu-MOF) and uses it to immobilize glucose oxidase. A multi-enzyme simulation system is established to achieve rapid glucose detection through a multi-enzyme cascade reaction.
Creative Enzymes offers professional services covering all glucose detection with enzymes to meet your research needs. With years of experience in nanozymes, our advanced platforms can help our clients solve various difficulties and conduct research. If you are interested in our services, please contact us for more details or make an online inquiry.