Enzyme Activity Measurement of Glucan Endo-1,3-Beta-D-Glucosidase

Creative Enzymes uses the top technologies and professional operations to give customers the best experience of our enzyme activity assays. We gained positive feedback from tens of thousands of completed service requests. The high-quality services and precise results have built our good reputation. We are proud to offer activity measurement for hydrolases such as glucan endo-1,3-beta-D-glucosidase.

Endo-1,3-beta-glucanases (EC 3.2.1.39) are members of the ubiquitous group of glycosidases, which are capable of hydrolyzing the glycosidic bond. The cleavage reaction of endo-1,3-beta-glucanases is limited to beta-1,3-glucosidic linkages present in unbranched segments consisting of several beta-1,3-linked glucosyl residues. The natural substrates of endo-1,3-beta-glucanases have a complex structural form consisting of a triple helix, such as curdlan. In addition, the enzyme also hydrolyzes laminarin, paramylon, and pachyman. The products of the hydrolysis reaction are (1→3)-beta-D-oligo glucosides ranging in length from two to nine glucose moieties, the vast majority of which are trisaccharides and tetrasaccharides.

Endo-1,3-beta-glucanases have been implicated in various physiological roles. For instance, in viruses, they are predicted to be involved in the degradation of the host cell wall during virus egress or entry. Endo-1,3-beta-glucanases present in archaea play a role during the fermentation process, while the bacterial enzymes have been shown to have lytic activity against fungi and metabolic functions. In the animal kingdom, endo-1,3-beta-glucanases are restricted to some invertebrates. For example, the enzymes expressed in nematodes allow them to feed on fungi, while in algae they are involved in the digestion of storage polysaccharides. This enzyme has various applications in agriculture, biotechnology, and food industries. For example, it seems to be a useful tool to prevent slime production and undesirable yeast growth during vinification. The extensive applications greatly stimulated studies on this enzyme. Since activity measurement is the first and necessary step of enzymology analysis, Creative Enzymes provides accurate activity assay service for endo-1,3-beta-glucanase to support such studies. The enzyme activity is determined by detecting the released glucose.

Figure: The crystal structure of endo-1,3-beta-glucanase from potato.
Reference: Wojtkowiak, A. et al. Acta Crystallogr D Biol Crystallogr. 2012 68: 713-723.

Based on the cutting-edge instrument and the skillful technologies, Creative Enzymes is able to deliver the most reliable results of testing endo-1,3-beta-glucanase. In order to offer the best-quality assay services, Creative Enzymes will continue learning the latest development and making progress in enzyme assays.