Enzyme Activity Measurement for Beta-Glucuronidase

After years of discovering and developing enzyme assays, Creative Enzymes has emerged as a worldwide leader in the field of activity measurement services. Creative Enzymes has continued growing with the same entrepreneurial and innovative spirit bringing science to commercialization. We firmly believe that the quality of our work and our services is the essential requirement for our high-level customer satisfaction. Creative Enzymes offers the most reliable enzymatic assays for hydrolases such as beta-glucuronidase.

Beta-glucuronidase (EC 3.2.1.31; beta-D-glucuronoside glucuronosohydrolase) is a ubiquitous protein involved in the degradation of glycosaminoglycans and glycoproteins in a wide range of cells of vertebrate and invertebrate tissues. This enzyme specifically hydrolyzes β-linked D-glucuronides to D-glucuronic acid and aglycones.

Functionally, the role of beta-glucuronidase in the migration of mesenchymal cells, the processes of cell-cell recognition, the control of cell growth, cell adhesion, cancer progression and normal cell development, has been well studied in the mammalian system. Moreover, this enzyme is implicated in diverse physiological and pathological processes and therefore is a potential target for the development of drugs, especially in the mediation of drug release.

Furthermore, even a wider range of applications of beta-glucuronidase indicate its promising future, which are seen in the determination of steroid levels in patients, the study of drug metabolism, the structural analysis of glycoproteins, glycosaminoglycans, proteoglycans, models in the differentiation of cells, and also in the oncogenic process. To that end, an understanding of the structures and functions of beta-glucuronidases is of considerable importance, not only to better comprehend their natural roles, but as a pre-requisite to further engineering of such enzymes to modify their specificity and efficiency. In fact, Escherichia coli beta-glucuronidase has already served as a test bed for the development of technologies for directed evolution of protein function.

Thus, reliable activity assays are the key to elucidate the catalytic mechanism of beta-glucuronidase to further gain an insight in the design of new drugs. Creative Enzymes is completely capable of providing the most reliable activity measurement for beta-glucuronidase. The activity of beta-glucuronidase is measured by the spectrophotometric assay which is considered as the most cost-effective method for enzymatic activity quantification. Creative Enzymes surpass its competitors with a quick turnover of the activity assays in response to the request of either a typical activity determination or a customized task. In a word, Creative Enzymes is your best choice and will always look forward to long-term partnership with customers.

Figure: The crystal structure of full-length E. coli beta-glucuronidase.
PDB: 3K46