Creative Enzymes is the leading biotech company which offers contract services for enzyme activity measurement. The experimental operations are performed by our specialized enzymologists who have been working with enzymes for many years. Associated with advanced equipment and extensive experiences, Creative Enzymes can provide the best assays for alpha-amylase.

Alpha-amylases (1,4-R-glucan 4-glucanohydrolase, EC 3.2.1.1) are ubiquitous enzymes which catalyze the breakdown of amylose and amylopectin through the hydrolysis of internal alpha-1,4-glycosidic linkages with net retention of anomeric configuration, simultaneously with the production of glucose and maltose. These enzymes act on starch, glycogen and related polysaccharides and oligosaccharides in a random manner. It is the major form of amylase in human and mammals whereas in fungi this enzyme is extracellularly secreted.

Alpha-amylases have been exploited for several applications including the food industry, drug development, and analysis. In the food industry, alpha-amylase is an important industrial enzyme in brewing and alcohol production. In addition, it is utilized in high-fructose syrup production. In clinical studies, the inhibition of alpha-amylase can significantly reduce the post-prandial increase of blood glucose and therefore can be an important strategy in the management of blood glucose level in type 2 diabetic and borderline patients. In the analysis, the alpha-amylase from A. amylolyticus is shown to be used in a sensitive method to detect very low concentration of heavy metals in drinking water. The activity determination methods of alpha-amylase differ in these applications. The commonly used methods are starch iodine test and the Phadebas amylase test, which is the qualitative and quantitative test employed in many industries. To support the precise determination of alpha-amylase activity in a variety of fields, Creative Enzymes performs the reliable Phadebas amylase test which satisfies your research needs, industrial demands, or other application requirements.

Figure: The crystal structure of alpha-amylase from Homo sapiens.
Reference: Ramasubbu, N. et al. Acta Crystallogr D Biol Crystallogr. 1996 52:435-46.

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