Enzyme Activity Measurement of Isocitrate Dehydrogenase (NADP+) Using Spectrophotometric Assays

Creative Enzymes is specialized in general and customized enzyme activity assays. Having tested enzymatic activities of variety oxidoreductases in the past few years, Creative Enzymes has accumulated extensive and professional experiences. We can provide exact approaches to enzyme activity measurement, including isocitrate dehydrogenase (NADP⁺).

Isocitrate dehydrogenase (ICDH) is an enzyme in the tricarboxylic acid (TCA) cycle that catalyzes the dehydrogenation and the subsequent decarboxylation of isocitrate to α-ketoglutarate and CO₂. EC 1.1.1.42 is a NADP⁺-linked enzyme that is found in both mitochondria and cytoplasm. The enzyme from some species can also use NAD⁺ but much more slowly. Isocitrate dehydrogenase (NADP⁺) requires Mn²⁺ or Mg²⁺ for activity. Unlike EC 1.1.1.41 (isocitrate dehydrogenase (NAD⁺)), oxalosuccinate can be used as a substrate of this enzyme. The enzyme is also known as:

• oxalosuccinate decarboxylase;
• oxalsuccinic decarboxylase;
• isocitrate (NADP) dehydrogenase;
• isocitrate (nicotinamide adenine dinucleotide phosphate) dehydrogenase;
• NADP-specific isocitrate dehydrogenase;
• NADP-linked isocitrate dehydrogenase;
• NADP-dependent isocitrate dehydrogenase;
• NADP isocitric dehydrogenase;
• isocitrate dehydrogenase (NADP-dependent);
• NADP-dependent isocitric dehydrogenase;
• triphosphopyridine nucleotide-linked isocitrate dehydrogenase-oxalosuccinate carboxylase;
• NADP⁺-linked isocitrate dehydrogenase;
• IDH (ambiguous);
• dual-cofactor-specific isocitrate dehydrogenase;
• NADP⁺-ICDH;
• NADP⁺-IDH;
• IDP;
• IDP1;
• IDP2;
• IDP3

Isocitrate dehydrogenase (NADP⁺) catalyzes the important step in the tricarboxylic acid (TCA) cycle. IDH1 and IDH2 are two isoforms of isocitrate dehydrogenase (IDH) in human using NADP⁺ as the cofactor. These two enzymes are of great clinical significance. Specific mutations in IDH1 have been found in several brain tumors including astrocytoma, oligodendroglioma, and glioblastoma multiforme. Due to the important role in metabolism and the pivotal value in medical research, more and more studies have started on this enzyme. The increasing needs of accurate enzyme activity assay become a gap in biotechnology to be solved. To this end, Creative Enzymes brings the reliable accurate spectrophotometric assays to support research and development activities on the enzyme.

Figure: The crystal structure of NADP-dependent isocitrate dehydrogenase from porcine heart mitochondria.
Reference: Ceccarelli C et al. J.Biol.Chem 2002 277: 43454-43462

Creative Enzymes has the capability of providing pertinent methods in combination with the reality. The excellent techniques and professional specialists here guarantee reliable and reproducible results. Creative Enzymes will sustain the standard and efficient service to support your research in all applications.