Enzyme Activity Measurement of Glucan 1,4-Alpha-Maltohydrolase

Creative Enzymes has developed the most advanced techniques in enzyme activity measurement and will always support your pioneering research in a professional way. Our advantages included the in-depth study on science, skillful operations, and diligent sales service. We have strong abilities in hydrolase activity testing, including glucan 1,4-alpha-maltohydrolase.

Glucan 1,4-alpha-maltohydrolase (maltogenic amylase, EC 3.2.1.133) is a potential catalyst for malto-oligosaccharide production. It is an amylolytic enzyme from glycosyl hydrolase family 13 (GH13). The enzyme catalyzes the hydrolysis of 1,4-alpha-D-glucosidic linkages in polysaccharides so as to remove successive alpha-maltose residues from the non-reducing ends of the chains. In addition, maltogenic amylase also acts on starch and related oligosaccharides.

Oligosaccharides have attracted much attention due to their numerous health benefits and the improvement of the physicochemical properties of foods. Therefore, a progressive increase in the demand for oligosaccharides has promoted the need for efficient catalysis in production. Oligosaccharides can be synthesized chemically, but enzyme catalyzed hydrolysis is essential to many important industrial reactions, with enzymatic hydrolysis being the basis of both the liquefaction and saccharification progresses during the production of high-glucose syrups from corn starch. Hence, alpha-amylases family members are preferred in the industries such as textile and detergent field, including maltogenic amylase. Besides, this enzyme has applications in medicine modifications. For example, maltogenic amylase is utilized to increase the solubility of puerarin by transglycosylating, thus, to become a therapeutic agent of coronary heart disease, cardiac infarction, and alcoholism. The enzyme activity can be measured by the dinitrosalicylic acid (DNS) method, which relies on precise spectrophotometers. Creative Enzymes provides reliable and flexible activity assays based on our extensive experiences. We can customize rapid services with unbeatable price to meet your research demand to the greatest extent.

Figure: The crystal structure of maltogenic amylase from S.marinus.
Reference: Jung, T.Y. et al. J Biol Chem. 2012 287(11): 7979–7989.

Creative Enzymes is particular specialized in spectrophotometric assays for various enzymes. Having testified by thousands of customers, our high-quality services are highly welcomed by tens of thousands of scientists from the world. We will keep on progressing to bring the satisfactory service. In the future, Creative Enzymes will continue the prominent service to help your research and business.