Enzyme Activity Measurement for Carboxylesterase

From the inception as a supplier of excellent enzyme activity assays, Creative Enzymes has become a globally recognized leader in the development of specialized enzyme measurement methods. Our aim is to lower the cost of customized enzyme assays so that they are accessible to all researchers. In addition, the high quality of our test results sets us apart from competitors. Herein, Creative Enzymes is proud to provide the most accurate enzyme assays for carboxylesterase.

Carboxylesterases (EC 3.1.1.1; carboxylic-ester hydrolase) are metabolic enzymes that are found across all kingdoms. Most of these enzymes are part of the α/β hydrolase fold superfamily of proteins. These enzymes catalyze the hydrolysis of carboxylic esters into their corresponding alcohols and carboxylic acids. Members of the α/β hydrolase fold superfamily share a conserved core structure. Carboxylesterases contain a catalytic triad consisting of a serine, an acidic amino acid, and a histidine. Mutation of any one of these residues results in a non-functional enzyme. The catalytic triad forms a charge relay system to create a nucleophilic serine that can attack the ester substrate. Carboxylesterases have broad substrate specificity. Several highly homologous carboxylesterase isozymes that exhibit overlapping substrate specificities have been identified in rodents. Importantly, carboxylesterases from microsomes also catalyze the reactions that are naturally catalyzed by many other hydrolases.

Carboxylesterases metabolize numerous clinically useful drugs (e.g., capecitabine, meperidine) as well as the illicit compounds heroin and cocaine, and commercial pesticides. Consequently, the development of specific carboxylesterase inhibitors may have both therapeutic and commercial use. Therefore, to investigate the functional significance of carboxylesterases, it is essential to accurately monitor the activity of carboxylesterases.

Creative Enzymes has the advantages in the superb technology, professional knowledge, and the most advanced equipment, which can support your research in a integrity. The spectrophotometric assay is considered as the reliable and cost-effective method for activity quantification for carboxylesterases. Fully equipped with the most advanced spectrophotometric instruments, Creative Enzymes assures the performance of the activity assay for carboxylesterases. Overall, Creative Enzymes is well known to provide the great level of customer satisfaction and will be your trusty-worthy partner in research and development.

Figure: The crystal structure of carboxylesterase from Geobacillus stearothermophilus.
PDB: 1R1D