Enzyme Activity Measurement for Acyl-CoA Oxidase

Creative Enzymes is the leading company in the field of enzyme activity assays, and is well known to deliver the best customer satisfaction. We are proud to offer the results of enzyme activity measurement for acyl-CoA oxidase in an accurate and efficient way.

Acyl-CoA oxidases (EC 1.3.3.6) are a group of flavoenzymes containing one non-covalently bound FAD per subunit as the prosthetic group and belong to the super-family of oxidoreductases, specifically those acting on the CH-CH group of donor with oxygen as acceptor. The systematic name of this enzyme class is acyl-CoA:oxygen 2-oxidoreductase, which is also known as fatty acyl-CoA oxidase, acyl coenzyme A oxidase or fatty acyl-coenzyme A oxidase.

The central pathway for fatty acid breakdown in higher plants is via peroxisomal β-oxidation. Fatty acids enter the cycle in the form of acyl-CoA thioesters, and during one round of β-oxidation, the acyl chain is shortened by a two-carbon unit and one acetyl-CoA molecule is produced. The first step in the peroxisomal β-oxidation cycle is the introduction of a double bond into the acyl-CoA substrate, resulting in the formation of 2-trans-enoyl-CoA. This reaction catalyzed by acyl-CoA oxidases is a two-step reaction. The cofactor FAD in this reaction gets reduced to its FADH- form in the first half-reaction concomitant with acyl-CoA oxidation. FADH- is then reoxidized by molecular oxygen in the second half-reaction, generating H₂O₂, a potential signaling molecule. Note that the members of the acyl-CoA oxidases family have different chain-length specificity for the acyl-CoA substrate, but they encompass the oxidation of the full range of acyl chains together present in vivo. In addition, acyl-CoA oxidases play a crucial role in many pathways, which include fatty acid degradation, alpha-Linolenic acid metabolism, biosynthesis of unsaturated fatty acids, biosynthesis of secondary metabolites and some other metabolic pathways.

Moreover, acyl-CoA oxidases serve the important role in biosynthesis, diagnostics and medical research. For instance, acyl-CoA oxidases are the essential enzymes in the biosynthesis of jasmonic acid, a signaling molecule involved in wound-induced plant defense. Acyl-CoA oxidases can also be used in diagnostic tests for the determination of free fatty acids in combination with acetate-CoA ligase. Owing to the wide applications of this enzyme in many fields, it is imperative to gain more detailed information about these enzymes, especially through activity measurements. Thus, Creative Enzymes is proud to provide the most precise and reliable catalytic activity quantification for acyl-CoA oxidases. Acyl-CoA oxidase activities can be determined at 420 nm and 30°C by measuring the formation of H₂O₂ spectrophotometrically. In a word, Creative Enzymes promises to sustain the professional standard and efficient services to stay as your trustworthy partner.

Figure: The crystal structure of acyl-CoA oxidase 1 from Arabidopsis Thaliana.
PDB: 1W07