Description
In enzymology, an acyl-CoA oxidase (EC 1.3.3.6) is an enzyme that catalyzes the chemical reaction acyl-CoA + O2↔ trans-2, 3-dehydroacyl-CoA + H2O2. Thus, the two substrates of this enzyme are acyl-CoA and O2, whereas its two products are trans-2, 3-dehydroacyl-CoA and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-CH group of donor with oxygen as acceptor. This enzyme participates in 3 metabolic pathways: fatty acid metabolism, polyunsaturated fatty acid biosynthesis, and ppar signaling pathway. It employs one cofactor, FAD.
Form
Yellow powder, lyophilized
Enzyme Commission Number
EC 1.3.3.6
Molecular Weight
78 kDa (SDS-PAGE)
pH Stability
6.0~8.5 (25°C, 15hr)
Michaelis Constant
10^-5 M (Palmitoyl-CoA)
Unit Definition
One unit will convert one micromole of Acyl-CoA to trans-2,3-dehydroacyl-CoAper min at pH 7.5 at 37°C.
Optimum temperature
37~40°C
Thermal stability
< 45°C (pH 7.5, 15min)
Inhibitors
Ag+, Hg2+, Zn2+, Cu2+, Ni2+
Synonyms
acyl-CoA oxidase; EC 1.3.3.6; fatty acyl-CoA oxidase; acyl coenzyme A oxidase; fatty acyl-coenzyme A oxidase; ACO
Notes
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
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