Description
In enzymology, an acyl-CoA oxidase (EC 1.3.3.6) is an enzyme that catalyzes the chemical reaction acyl-CoA + O2↔ trans-2, 3-dehydroacyl-CoA + H2O2. Thus, the two substrates of this enzyme are acyl-CoA and O2, whereas its two products are trans-2, 3-dehydroacyl-CoA and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-CH group of donor with oxygen as acceptor. This enzyme participates in 3 metabolic pathways: fatty acid metabolism, polyunsaturated fatty acid biosynthesis, and ppar signaling pathway. It employs one cofactor, FAD.
Abbr
Acyl-CoA oxidase (Arthrobacter sp.)
Product Overview
Native acyl-CoA oxidase (EC 1.3.3.6) was purified from Arthrobacter sp..
Form
Yellowish Freeze dried powder
Enzyme Commission Number
EC 1.3.3.6
Contaminants
Catalase < 1.00%; Glucose oxidase < 0.01%
pH Stability
6.0-7.5 (37°C, 60 mins)
Thermal stability
Stable at 40°C and below (pH 7.0, 10 mins)
Storage
Store in tightly closed containers, desiccated, protected from light, at-20°C.
Preparation Instructions
Useful for enzymatic determination of fatty acid when coupled with Acyl-CoA synthetase
Synonyms
acyl-CoA oxidase; EC 1.3.3.6; fatty acyl-CoA oxidase; acyl coenzyme A oxidase; and fatty acyl-coenzyme A oxidase
Download Datasheet