Enzyme Activity Measurement of IMP Dehydrogenase Using Spectrophotometric Assays

Creative Enzymes has presented a high level of service quality and enjoyed a good reputation since the service of enzyme activity assays were introduced to the industry. We are equipped with professional techniques and advanced instruments, and backed by superb scientist teams with extensive experiences. We are specialized in oxidoreductase activity assay, such as IMP dehydrogenase.

IMP dehydrogenases belongs to the oxidoreductase family. This enzyme converts inosine 5'-phosphate to produce xanthosine 5'-phosphate, using NAD⁺ as a cofactor. It is also known as:

• Inosine-5'-monophosphate dehydrogenase
• Inosinic acid dehydrogenaseis
• IMPDH

Inosine monophosphate (IMP) is produced by de novo purine nucleotide biosynthesis. It is the precursor to both adenine and guanine nucleotides, essential materials for DNA and RNA synthesis. The enzyme IMPDH owns a primary position in virtually every organism. It controls the sole biosynthetic route to guanine nucleotides. Therefore IMP dehydrogenase is extremely important due to its biological significance. The reaction catalyzed by IMPDH is the first committed and rate-limiting step in guanine nucleotide biosynthesis. In humans and other mammals, the enzyme is encoded by two IMPDH genes, IMPDH1 and IMPDH2. In general, human IMPDH1 is expressed constitutively at low levels, while IMPDH2 is amplified only during proliferation and transformation. More specifically, rapidly growing cells have a high demand for guanine nucleotides that generally cannot be sustained by salvage pathways. As a result, the synthesis pathway needs to be activated to supply higher-than-regular amounts of guanine nucleotides, which further explains the key role of IMPDH in cancer and viral infection. The enzyme then evidently has emerged as an attractive target for antiparasitic, antiviral, antibacterial, and immunosuppressive therapies. Hence, IMPDHs from different organisms have been biochemically and structurally characterized, hoping to serve their therapeutic value. In fact, recent studies have already suggested that methiopropamine (MPA) and benzamide riboside are two of its inhibitors. Considering the increasing assay demands for IMP dehydrogenase activity, Creative Enzymes supports biomedical research with the most advanced spectrophotometric tests. The enzymatic activity can be detected at 260 nm or 340nm, due to the consumption of NAD⁺ or generation of NADH.

Figure: The crystal structure of the IMP dehydrogenase from Ashbya gossypii.
Reference: Rubén M. Buey et al. Appl Microbiol Biotechnol. 2015 99:9577-89

Creative Enzymes is one of the very few companies providing the comprehensive assay service for IMP dehydrogenase. Based on our extensive experiences and knowledgeable specialists, we are able to provide exact research plan according to every customers’ requests. For any research purposes, Creative Enzymes will always be your best choice for enzyme activity testing.