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Creative enzyme is committed to providing customers with high-quality enzyme activity assay services. We are proud of our professional technology, excellent team, and fast and efficient detection of enzyme activity, including D-lactate dehydrogenase.
D-lactate dehydrogenases and L-lactate dehydrogenases (D-LDH and L-LDH) reduce pyruvate to D- and L-lactate, respectively, with oxidization of NADH into NAD+, and act as the last step of the glycolytic pathway under anaerobic conditions. D-lactate dehydrogenase is also known as:
D-LDHs belongs to a large D-2-hydroxyacid dehydrogenase (D-HydDH) superfamily, which comprises various types of D-isomer dehydrogenases, such as the D-glycerate, D-3-phosphoglycerate, D-2-hydroxyglutarate and D-2-hydroxyisocaproate dehydrogenases. Although these enzymes bear highly divergent primary sequences, their tertiary structures are quite similar to one another. The Lactobacillus D-LDHs consistently have a dimeric structure comprising identical subunits, and exhibit hyperbolic shaped saturation curves for substrate pyruvate. And in E. coli cells, D-LDH gives a homotetrameric structure. Bacterial allosteric D-LDHs are commonly activated by FBP (fructose 1,6-bisphosphate) and the inhibition occurres when the oxmate presents in the solvent. D-lactate dehydrogenases activity was determined by measuring the variation of the absorbance at 340 nm derived from NADH. Creative Enzymes offers rapid and accurate assay methods to detect not only purified D-lactate dehydrogenases, but also samples in serum, plasma, cell lysates, culture and fermentation media.
Figure: The crystal structure of D-lactate dehydrogenase from Escherichia coli.
Reference: Furukawa et al. AMB Express 2014, 4:76
Creative Enzymes is exceptionally skilled at providing assay services and proficient in activity measurement using spectrophotometry, or UV/vis spectroscopy in this case. Professional techniques and extensive experiences support us stand at the forefront of the enzyme activity assay industry. In the future, Creative Enzymes will continue to be the reliable partner in every aspect of your need with activity assays.
