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D-lactate Dehydrogenase from E. coli, Recombinant
Cat No.
NATE-1654
Description
In enzymology, a D-lactate dehydrogenase is an enzyme that catalyzes the chemical reaction: (D)-lactate + 2 ferricytochrome c↔ pyruvate + 2 ferrocytochrome c. Thus, the two substrates of this enzyme are (D)-lactate and ferricytochrome c, whereas its two products are pyruvate and ferrocytochrome c. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with a cytochrome as acceptor. This enzyme participates in pyruvate metabolism. It employs one cofactor, FAD.
Abbr
D-LDH, Recombinant (E. coli)
Source
E. coli
Product Overview
This protein is fused with 6x His tag at N terminus and the protein has a calculated MW of 39.1 kDa (353aa).
Form
Liquid
Enzyme Commission Number
EC 1.1.1.28
Activity
> 200 units/mg
Molecular Weight
39.1 kDa
Purity
> 95% by SDS-PAGE
Concentration
1 mg/ml
Unit Definition
One unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37°C.
Storage
Store at +4°C for short term (1-2 weeks). For long term storage, aliquot and store at -70°C. Avoid repeated freeze/thaw cycles.
Synonyms
EC 1.1.1.28; D-Lactic Dehydrogenase; 9028-36-8; (D)-lactate:ferricytochrome-c 2-oxidoreductase; lactic acid dehydrogenase; D-lactate (cytochrome) dehydrogenase; cytochrome-dependent D- (−)-lactate dehydrogenase; D-lactate-cytochrome c reductase; D- (−)-lactic cytochrome c reductase
Formulation
4.0 mg/ml in 20 mM potassium phosphate, 100 mM NaCl, pH 8.0 and 10% glycerol
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-0195 | Native Lactobacillus leichmanii D-Lactic Dehydrogenase | EC 1.1.1.28 | 9028-36-8 | Lactobacillus leichmanii | Inquiry |
| NATE-0196 | Native Leuconostoc mesenteroides D-Lactic Dehydrogenase | EC 1.1.1.28 | 9028-36-8 | Leuconostoc mesenteroides | Inquiry |
| NATE-0197 | Native Staphylococcus epidermidis D-Lactic Dehydrogenase | EC 1.1.1.28 | 9028-36-8 | Staphylococcus epidermidis | Inquiry |
| NATE-0976 | Native Lactobacillus delbrückii D-Lactate Dehydrogenase, Grade I | 9028-36-8 | Lactobacillus delbrückii | Inquiry |