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D-lactate Dehydrogenase from E. coli, Recombinant

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Cat No.

NATE-1654

Description

In enzymology, a D-lactate dehydrogenase is an enzyme that catalyzes the chemical reaction: (D)-lactate + 2 ferricytochrome c↔ pyruvate + 2 ferrocytochrome c. Thus, the two substrates of this enzyme are (D)-lactate and ferricytochrome c, whereas its two products are pyruvate and ferrocytochrome c. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with a cytochrome as acceptor. This enzyme participates in pyruvate metabolism. It employs one cofactor, FAD.

Abbr

D-LDH, Recombinant (E. coli)

Source

E. coli

Product Overview

This protein is fused with 6x His tag at N terminus and the protein has a calculated MW of 39.1 kDa (353aa).

Form

Liquid

Enzyme Commission Number

EC 1.1.1.28

Activity

> 200 units/mg

Molecular Weight

39.1 kDa

Purity

> 95% by SDS-PAGE

Concentration

1 mg/ml

Unit Definition

One unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37°C.

Storage

Store at +4°C for short term (1-2 weeks). For long term storage, aliquot and store at -70°C. Avoid repeated freeze/thaw cycles.

Synonyms

EC 1.1.1.28; D-Lactic Dehydrogenase; 9028-36-8; (D)-lactate:ferricytochrome-c 2-oxidoreductase; lactic acid dehydrogenase; D-lactate (cytochrome) dehydrogenase; cytochrome-dependent D- (−)-lactate dehydrogenase; D-lactate-cytochrome c reductase; D- (−)-lactic cytochrome c reductase

Formulation

4.0 mg/ml in 20 mM potassium phosphate, 100 mM NaCl, pH 8.0 and 10% glycerol

"D-LDH" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0195	Native Lactobacillus leichmanii D-Lactic Dehydrogenase	EC 1.1.1.28	9028-36-8	Lactobacillus leichmanii	Inquiry
NATE-0196	Native Leuconostoc mesenteroides D-Lactic Dehydrogenase	EC 1.1.1.28	9028-36-8	Leuconostoc mesenteroides	Inquiry
NATE-0197	Native Staphylococcus epidermidis D-Lactic Dehydrogenase	EC 1.1.1.28	9028-36-8	Staphylococcus epidermidis	Inquiry
NATE-0976	Native Lactobacillus delbrückii D-Lactate Dehydrogenase, Grade I		9028-36-8	Lactobacillus delbrückii	Inquiry

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