Enzyme Activity Measurement for Sugar-Phosphatase

Creative Enzymes is proud to provide spectrophotometric activity assays for sugar-phosphatase. We are a group of scientists who have been working on enzyme activity assays for years. The quality of our results is ensured by the most advanced spectrophotometric instruments and our state-of-the-art technology. Our passion for high quality and unique services is only exceeded by our excellent reputation in the marketplace.

Sugar-phosphatase (EC 3.1.3.23; sugar-phosphate phosphohydrolase) is an enzyme that catalyzes the hydrolysis of the phosphate esters of sugars. Sugar-phosphatase is located exclusively in the periplasmic space membrane of bacteria. This enzyme can be purified from various bacteria, such as Escherichia coli, Neisseria meningitidis, and Lactococcus lactis. The sugar-phosphatases from E. coli and N. meningitidis are very similar to each other in terms of substrate specificity, intracellular localization, effects of glucose on enzyme biosynthesis, and physicochemical and catalytic properties. Sugar-phosphatase belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. Note that this enzyme has a wide substrate specificity, which can act on aldohexose 1-phosphates, ketohexose 1-phosphates, aldohexose 6-phosphates, ketohexose 6-phosphates, both phosphate ester bonds of fructose 1,6-bisphosphate, phosphoric esters of disaccharides, and pentose and triose phosphates.

Sugar-phosphatase may be a unique homologous enzyme present in certain microorganisms which share a common system of transport and metabolism of sugars and phosphates. Besides, sugar-phosphatase may be the enzyme exploited for the cleavage of phosphorylated sugars transported via either the phosphoenolpyruvate-hexose phosphotransferase or other systems. Thus, to understand the physiologic role of this enzyme and its relationship with the phosphoenolpyruvate-hexose phosphotsansferase system, it is indispensable to be able to detect and monitor the activity of sugar-phosphatase.

Creative Enzymes is honored to offer the accurate enzyme activity assay for sugar-phosphatase by using spectrophotometric assay, which is considered to be the most reliable and cost-effective method for activity quantification. Fully equipped with the most advanced spectrophotometric instruments, we promise to return the test results in a professional and timely manner. With decades of experiences and our unique technologies, Creative Enzymes prides itself on its commitment to providing its customers with the highest-quality services covering pharmaceutical, biotechnological and diagnostic industries.

Figure: The crystal structure of Escherichia coli sugar-phosphatase SUPH.
PDB: 2HF2