Description
In enzymology, a sugar-phosphatase (EC 3.1.3.23) is an enzyme that catalyzes the chemical reaction: sugar phosphate + H2O ↔ sugar + phosphate. Thus, the two substrates of this enzyme are sugar phosphate and H2O, whereas its two products are sugar and phosphate. This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. The systematic name of this enzyme class is sugar-phosphate phosphohydrolase.
Abbr
Sugar-phosphatase, Recombinant (E. coli)
Source
Escherichia coli str. K-12 substr. MG1655
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.1.3.23
Molecular Weight
34233.0 Da
Purity
>95 % as judged by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to release 1μmol of pNP per minute from pNP-phosphate (15.6 mM) in 78.1 mM sodium acetate buffer, pH 5.5, containing 0.064 mg/mL BSA and 6.25 mM MgCl2, at 40°C, and using an extinction coefficient of 18000 M-1cm-1.
Optimum temperature
> 40°C
Storage
Store at 4°C (shipped at room temperature)
Preparation Instructions
Agitate vial sufficiently to fully homogenise enzyme precipitate before use.
Synonyms
Sugar-phosphate phosphohydrolase
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