Enzyme Activity Measurement for Alcohol Oxidase

Creative Enzymes has already helped many professionals to achieve enzyme activity measurement with efficient and accurate services. Our passion for high quality and unique service is only exceeded by our excellent reputation in the marketplace. Our superb team will constantly improve in conducting the measurement of alcohol oxidase activity.

Alcohol oxidase (EC 1.1.3.13; AO) is a 600 kDa homo-octameric enzyme, which has eight identical subunits. Each subunit contains a strongly and non-covalently bound flavin adenine nucleotide (FAD) molecule as the prosthetic group. This enzyme has been isolated and charactrized from a variety of yeasts and bacteria, such as Pichia pastoris, Paecilomyces variotii, Pichia methanolica, and Thermoascus aurantiacus. Alcohol oxidase is the key enzyme of methanol metabolism in methylotrophic yeast species. It catalyzes the first step of methanol metabolism. Thus alcohol oxidase is a rapid and efficient catalyst for oxidation of lower primary alcohols to the corresponding aldehydes using a two-step mechanism: first a reductive half-reaction, where the sustrate is oxidized while FAD is reduced to FADH₂, followed by an oxidative half-reaction, where the flavin is reoxidized by O₂ and H₂O₂ is produced. This enzyme is classified as a member of the glucose-methanol-choline (GMC) oxidoreductase family, and the systematic name of this enzyme class is alcohol:oxygen oxidoreductase, which is also called ethanol oxidase.

Figure: The crystal structure of alcohol oxidase from Pichia pastoris.
PDB: 5I68

The detection and quantification of alcohols are important in the routine analysis of various fields such as food industry, medical science, agriculture, and the environment applications. Ethanol biosensors based on alcohol oxidase have been reported to be stable and practical. To improve the operational potential of alcohol oxidase, studies on immobilization methods, enzymatic reaction kinetics, operation modes of biosensors, and the catalytic mechanisms have become more and more popular. Hence, Creative Enzymes is proud to make the accurate activity assay available for alcohol oxidase, based on years of extensive experiences on testing the enzymes and optimizing the method. Spectrophotometric assays for alcohol oxidase activities are performed by following the absorbance at 420 nm, indicating formation of H₂O₂. Overall, Creative Enzymes is enthusiastic in providing accurate enzyme activity assays. We are sincerely looking forward to building solid partnership with clients. Our prompt service, good customer care, and dedicated approaches have made us the most preferred provider of enzyme assays.