Description
In enzymology, an alcohol oxidase (EC 1.1.3.13) is an enzyme that catalyzes the chemical reaction:a primary alcohol + O2↔ an aldehyde + H2O2. Thus, the two substRates of this enzyme are primary alcohol and O2, whereas its two products are aldehyde and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. It employs one cofactor, FAD.
Abbr
Alcohol Oxidase, Native (Pichia pastoris)
Alias
alcohol oxidase; ethanol oxidase
Applications
Alcohol Oxidase may be used to study protein translocation into peroxisomes. This product is from Pichia pastoris. It has been used for the bacterial expression and immunological verification of Hv-p68 cDNA clones.
Form
Buffered aqueous solution. Solution in 30% sucrose with 0.1 M phosphate buffer at pH 8.0
Enzyme Commission Number
EC 1.1.3.13
Activity
10-40 units/mg protein (biuret)
Unit Definition
One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25°C.
Synonyms
EC 1.1.3.13; 9073-63-6; alcohol oxidase; ethanol oxidase; Alcohol:oxygen oxidoreductase