Creative Enzymes is a leading company in the field of enzymes activity measurement. With the support of an enzymology expert team and the power of a state-of-the-art laboratory, we are able to perform spectrophotometric assays for activity quantification of 12 alpha-hydroxysteroid dehydrogenase. The reliability of the result is based on our most advanced spectrophotometric instruments and the well-developed method.

12 Alpha-hydroxysteroid dehydrogenase (EC 1.1.1.176) belongs to the family of oxidoreductases. More precisely, it is a member of the oxidoreductases that act on the CH-OH group of the electron donor with NAD⁺ (nicotinamide adenine dinucleotide) or NADP⁺ (nicotinamide adenine dinucleotide phosphate) as the electron acceptor. In metabolism, 12 alpha-hydroxysteroid dehydrogenase degrades 12 alpha-hydroxy group of bile acids, as well as bile alcohols, into cholesterols. In enzymology, the enzyme catalyzes the oxidation of 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholanate and NADP⁺ into 3 alpha,7 alpha-dihydroxy-12-oxo-5 beta-cholanate with NADPH and H⁺. The systematic name of this enzyme class is 12 alpha-hydroxysteroid:NADP⁺ 12-oxidoreductase, and it was also named as:

• 12alpha-hydroxy steroid dehydrogenase;
• NAD+-dependent 12alpha-hydroxysteroid dehydrogenase;
• NADP+-12alpha-hydroxysteroid dehydrogenase

12 Alpha-hydroxysteroid dehydrogenase is NAD-dependent and active against conjugated and unconjugated forms of bile salts. It is involved in the metabolism of bile salts and cholesterol. Cholesterol is the essential component of cell membranes structure to maintain its integrity and fluidity, and the precursor for the steroid hormones and bile acids biosynthesis. In addition, the 12alpha-hydroxysteroid dehydrogenase, which is heat labile, together with 3alpha-hydroxysteroid dehydrogenases, which is heat stable, could be applied in the spectrophotometric quantification of bile salts.

Creative Enzymes is your trustworthy partner for activity measurement for 12 alpha-hydroxysteroid dehydrogenase. We always impress customers with good sincere, dedicated services, and precise and reliable measurement results. Therefore, choosing Creative Enzymes can help you remove anxiety of enzyme activity measurements for 12 alpha-hydroxysteroid dehydrogenase. Your request will be well taken care of by our strong enzymologist team with the most advanced spectrophotometric instrument.

Figure: Molecule diagrams of the chemical reaction catalyzed by 12alpha-hydroxysteroid dehydrogenase.