Enzyme Activity Measurement of UTP-Monosaccharide-1-Phosphate Uridylyltransferase

Creative Enzymes has rapidly grown to a top-class enzyme service supplier. In the past several years, we have established a testing platform and standard system based on analytical instruments of different sensitivity levels. Our high-quality activity assays for UTP-monosaccharide-1-phosphate uridylyltransferase are guaranteed with accurate results.

UTP-monosaccharide-1-phosphate uridylyltransferase (PsUSP, EC 2.7.7.64) is a unique nucleotide-sugar pyrophosphorylase that can utilize diverse sugar 1-phosphates to form their corresponding UDP-sugars. The available substrates include glucose 1-phosphate, galactose 1-phosphate, glucuronic acid 1-phosphate, and xylose 1-phosphate, whereas mannose 1-phosphate, L-fucose 1-phosphate, and glucose 6-phosphate do not serve as substrates. The enzymatic reaction is limited to using UTP rather than other nucleotide triphosphates. PsUSP requires Mg²⁺ or Mn²⁺ for maximal activity.

PsUSP is important for the biosynthesis of activated carbohydrates which play pivotal roles in a vast amount of cellular processes. Activated carbohydrates participate in glycoprotein biosynthesis, cellular responses, signal transductions, and sucrose and polysaccharide metabolisms in plants. Moreover, they are related to the surface modification of cell walls or even the complete disguise of organisms by forming a complex protective glycocalyx coat. Such camouflage strategy contributes to escaping the recognition by an infected host. Every single year, approximately 12 million people are infected by protozoan parasites and 60,000 people died of it. Therefore, PsUSP has become an attractive target for the development of anti-parasite drugs. The published crystal structure of PsUSP from Leishmania major provides an insight into the catalytic mechanism. Based on the continuous studies on this enzyme, the efficient medicines are hopefully to be developed. To facilitate such an effort, the experienced experts in Creative Enzymes developed proper testing approaches towards different research activities. The activity assays are performed with the most reliable chromatographic assays.

Figure: The crystal structure of USP from L. major bound to uridine-5'-triphosphate.
Reference: Dickmanns, A. et al. J. Mol. Biol. 2011 405: 461-478.

Creative Enzymes is one of the few companies that are able to provide activity assay services for UTP-monosaccharide-1-phosphate uridylyltransferase. Our exceptional ability and superior service quality are endorsed by more and more customers. With the goal to provide the best assay services for transferases, we will never stop updating the expertise and elevating the technology.