Enzyme Activity Measurement of Malate Dehydrogenase (Oxaloacetate-Decarboxylating) (NADP+) Using Spectrophotometric Assays

Creative Enzymes is professional in providing accurate and high-quality activity measurement services. Having the most advanced instrument and technical team, Creative Enzymes can perform enzymatic activity testing in a reliable and efficient way, including measurement of oxidoreductases such as malate dehydrogenase (decarboxylating) (NADP⁺).

Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP⁺) belongs to the family of oxidoreductases and catalyzes the oxidative decarboxylation of (S)-malate in the presence of NADP⁺ and divalent metal ions, such as Mg²⁺ or Mn²⁺. It can also catalyze the decarboxylation of oxaloacetate which differs from EC 1.1.1.39. Its alternative names are:

• malic enzyme (ambiguous) 003B
• pyruvic-malic carboxylase (ambiguous);
• malate dehydrogenase (decarboxylating, NADP+);
• NADP⁺-linked decarboxylating malic enzyme;
• NADP⁺-malic enzyme;
• NADP⁺-specific malic enzyme;
• NADP⁺-specific malate dehydrogenase;
• malate dehydrogenase (NADP⁺, decarboxylating);
• L-malate:NADP⁺ oxidoreductase

Regarding its wide distribution in biological species, malate dehydrogenase (decarboxylating) (NADP⁺) exists in a broad range of eukaryotic organisms, from fungi to mammals. Malic enzymes are the core component of many important biological functions. In plant, NADP-malic enzyme is one of the three decarboxylation enzymes used in the inorganic carbon concentrating mechanisms of C4 and CAM plants. In fungi and other organisms, a malic enzyme, via NADPH production, is believed to have lipogenic functions. Similarly, the cytosolic NADP⁺-dependent isoform of malic enzyme (cNADP-ME) in mammals is involved in the generation of NADPH and the biosynthesis of fatty acids and steroids in liver and adipose tissues. In addition, cNADP-ME may also have a role in microsomal drug detoxification. More and more researches on malate dehydrogenase (decarboxylating) (NADP⁺) have been initiated due to the pivotal position it holds in biology. The increasing needs of accurate enzyme activity assays become a gap in biotechnology to be solved. To this end, Creative Enzymes brings the reliable spectrophotometric assay services to support research and development activities on it.

Figure: The crystal structure of human NADP-dependent malic enzyme.

Creative Enzymes in the leading service company in the enzyme assay industry. The advanced equipment and experienced specialists here will support your research in a timely and effective way. In the future, Creative Enzymes will continue to be the cornerstone of your success.