Creative Enzymes has presented a professional level of service quality and enjoyed a good reputation in the enzyme activity assay industry. Our extensive experiences in providing fast and effective testing method guarantee accurate assay results. Testing oxidoreductases is one of our advantages, including the measurement of hydroxymethylglutaryl-CoA reductase (NADPH).

Hydroxymethylglutaryl-CoA reductase (NADPH) catalyzes the formation of mevalonate, the committed step in the biosynthesis of sterols and isoprenoids. It is also known as:

• hydroxymethylglutaryl coenzyme A reductase (reduced nicotinamide adenine dinucleotide phosphate)
• 3-hydroxy-3-methylglutaryl-CoA reductase
• β-hydroxy-β-methylglutaryl coenzyme A reductase
• hydroxymethylglutaryl CoA reductase (NADPH)
• S-3-hydroxy-3-methylglutaryl-CoA reductase
• NADPH-hydroxymethylglutaryl-CoA reductase
• HMGCoA reductase-mevalonate:NADP-oxidoreductase (acetylating-CoA)
• 3-hydroxy-3-methylglutaryl CoA reductase (NADPH)
• hydroxymethylglutaryl-CoA reductase (NADPH2).

Sequence comparison among HMGR variants revealed two distinct classes: eukaryotic HMGRs (class I) and prokaryotic HMGRs (class II). Except the archeal members, all class I HMGRs contain N–terminal membrane domains, while class II HMGRs lack a membrane domain and are soluble.

Figure: The complex of the catalytic portion of human hydroxymethylglutaryl-CoA reductase (NADPH) with HMG, CoA, and NADP+.

This enzyme also participates in important biosynthesis pathways. Mevalonic acid is the precursor of isoprenoids, a class of compounds involved in diverse cellular functions such as sterol synthesis and growth control. Products of the mevalonate pathway include cholesterol, heme and farnesyl-pyrophosphate. The activity of HMGR is controlled through synthesis, degradation, and phosphorylation to maintain the concentration of mevalonate-derived products. In addition to the physiological regulation of HMGR, the human enzyme has been targeted successfully by drugs in the clinical treatment of high serum cholesterol levels. Due to the increasingly important position in research and therapeutic uses, the studies on hydroxymethylglutaryl-CoA reductase (NADPH) have gained growing attention. Thus, Creative Enzymes would like to provide high-quality activity measurement services of hydroxymethylglutaryl-CoA reductase (NADPH) to meet the industrial needs in all applications with an accurate and precise result.

Creative Enzymes is the leading service institution that provides reliable tests. Our test results are in exceedingly high quality among the industry. The advanced equipment and experienced specialists here will support your research in a timely and effective way. Creative Enzymes will sustain the professional standard to continue to support your research activities.