Enzyme Activity Measurement of Glycoprotein-Fucosylgalactoside Alpha-N-acetylgalactosaminyltransferase

Creative Enzymes stands out among a great number of enzyme service companies, relying on the extraordinary technologies and specialized expertise. Judging by the service quality, our activity assays have exceeded the majority of the enzyme industry. Our accurate measurement on transferases are testified by tens of thousands of customers. Especially we offer a reliable test method for glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase.

Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase (EC 2.4.1.40) belongs to the class of glycosyltransferases (GT), which synthesize new glycosidic linkages by adding monosaccharide units from activated donor molecules such as UDPGal in a stereospecific and regiospecific manner to target acceptor molecules. EC 2.4.1.40 specifically transports the hexosyl group from UDP-N-acetyl-alpha-D-galactosamine to glycoprotein-alpha-L-fucosyl-(1,2)-D-galactose, releasing the UDP molecule. The catalytic reaction needs no cofactor but the participation of Mn²⁺ ion.

GT genes are ubiquitous in vertebrates. They have not been found in invertebrates, plantsyeast, or archaea. The GT genes in humans are involved in the generation of individual variations in blood group structures (A, B, AB, or O). EC 2.4.1.40 contributes to the formation of the blood group A antigen from the H-antigen and performs the final glycosylation step in A antigen synthesis. In addition to the lactoseries and neolactoseries of glycosphingolipid biosynthesis, this enzyme also participates in the biosynthesis of glycan structures such as polysaccharides and complex oligosaccharides, which have vital roles in biological structures and processes including cellular interactions, matrix structures, signaling, pathogenesis, and immunity. Even after years of studies on this enzyme, the approaches of activity measurement still greatly limits further applications of this enzyme. Complicated methods are not only time consuming but also give large variations in activity results. Creative Enzymes exploits a standard assay method that can rapidly and accurately measure the enzyme activity.

Figure: The complex structure of glycosyltransferase A with UDP and H antigen acceptor.
Reference: Patenaude SI et al. Nat Struct Biol. 2002 9(9): 685-90.

The advantages of services provided by Creative Enzymes include the excellent abilities in experiment operations and strict and meticulous service attitudes. Our activity measurement for EC 2.4.1.40 is equipped with high-efficient approaches and best performing facilities. The results are authentic and reliable to all researchers from different industries.