Creative Enzymes is no doubt a leader in the field of enzyme activity. We have the most advanced techniques and professional research team to provide high-quality services of enzymatic activity determination. At Creative Enzymes, the accurate and rapid assay service for delta4-3-oxosteroid 5beta-reductase generates the most accurate and reproducible results.

Delta4-3-oxosteroid 5beta-reductase is a soluble monomeric NADPH dependent enzyme and a member of the aldo-keto reductase (AKR) superfamily. Due to the essential role in primary bile acid and steroid hormone biosynthesis, delta4-3-oxosteroid 5beta-reductase has been well studied in mammals, especially in humans, where the enzyme is also designated as AKR1D1. AKR1D1 catalyzes the irreversible reduction of Δ4-3-ketosteroids to 5β-dihydrosteroids to introduce a 90° bend at the steroid A/B ring junction, using NADPH as the hydride donor. The other names of this enzyme include the followings:

• 3-oxo-Delta4-steroid 5beta-reductase;
• 5beta-reductase;
• androstenedione 5beta-reductase;
• cholestenone 5beta-reductase;
• cortisone 5beta-reductase;
• cortisone beta-reductase;
• cortisone Delta4-5beta-reductase;
• steroid 5beta-reductase;
• testosterone 5beta-reductase;
• Delta4-3-ketosteroid 5beta-reductase;
• Delta4-5beta-reductase;
• Delta4-hydrogenase;
• 4,5beta-dihydrocortisone:NADP⁺ Delta4-oxidoreductase;
• 3-oxo-5beta-steroid:NADP⁺ Delta4-oxidoreductase

In bile acid biosynthesis, the conversion of configuration catalyzed by this enzyme is a vital part. This cis-configuration is critical for bile acids to properly emulsify dietary fats and cholesterol, which is difficult to achieve by synthetic approaches. The defect of this enzyme will lead to liver failure and hemochroma tosis, conditions that are potentially lethal in newborn infants. In addition to being involved in bile acid biosynthesis, AKR1D1 is also responsible for generating 5β-pregnanes, which are natural ligands for the pregnane-X receptor (PXR) in the liver. PXR is involved in the induction of cytochrome P450 3A4 (CYP3A4), which is responsible for the metabolism of a large proportion of drugs. Considering the irreplaceable and potential roles in therapy, more studies have been carrying out on this enzyme. To meet the research need, Creative Enzymes provides proper activity assays that satisfy the unmet need of enzyme characterization. Our reliable spectrophotometric services is specially optimized to quantify the enzyme activity.

Figure: The crystal structure of 5beta-reductase (AKR1D1) E120H mutant in complex with NADP⁺.
Reference: Mo Chen et al. J.Biol.Chem. 2012, 287: 16609-16622

Creative Enzymes maintains a leading position in enzyme assay services, relying on the cutting-edge equipment and constantly updated knowledge. We provide high-quality assay services that significantly exceed the average level in this industry. Creative Enzymes are sincerely looking forward to learning about your specific requests and helping with our unique services.