Enzyme Activity Measurement of Blood-Group-Substance Endo-1,4-Beta-Galactosidase

Creative Enzymes is a biotechnology company and has been one of the most popular source of enzyme services. Our rapid and professional service have been testified by many customers from various industries. Our research center is equipped with all types of advanced analytical instruments, which is the basis of our accurate tests for different research requests. The experienced enzymologists will choose and develop the most suitable assay based on the detailed service request, and deliver reproducible results on time. The tests for blood-group-substance endo-1,4-beta-galactosidase have been one of the most satisfactory services.

Blood-group-substance endo-1,4-beta-galactosidase (EABase, EC 3.2.1.102) is the first discovered member of glycoside hydrolase family GH98, denoting its endoglycosidase activity on both A-antigen and B-antigen. More specifically, EABase catalyzes the hydrolysis the 1,4-beta-D-galactosyl linkages adjacent to 1,3-alpha-D-galactosyl or N-acetylgalactosaminyl residues, and a 1,2-alpha-D-fucosyl residue. EABase completely removes the whole trisaccharide of the A and B antigen from glycans on the host cell surface, leaving the antigen-null red blood cells. The main antigenic differences in AOB group system is that the consistence of trisaccharide moiety attached to glycoproteins and lipids on the surface of red blood cells. The trisaccharide of the A-antigen (GalNAcα-1,3-(Fucα-1,2)-Galβ-) terminates with an N-acetylgalactosamine, whereas, in the B-antigen (Galα-1,3-(Fucα-1,2)- Galβ-), the terminal sugar is galactose. Glycans of O-type cells lacking this terminal sugar thus present the core α-Fuc-1,2-ß-Gal- structure and are nonantigenic for the vast majority of individuals. Enzymatic removal of the terminal N-acetylgalactosamine or galactose of A- or B- antigens, respectively, to generate O-type blood has long been proposed as a means of converting erythrocytes into a universal and antigen-null donor type, which has significance in immune response research. Activity measurement for this enzyme is usually performed by fluorometric assays. Creative Enzymes provides activity assay services for EABase that satisfy your research on enzyme kinetics, substrate specificity, and mutant enzymes evaluation.

Figure: The Crystal structure of blood-group-substance endo-1,4-beta-galactosidase from Streptococcus pneumoniae.
Reference: Kwan, D.H. et al. J. Am. Chem. Soc. 2015 137: 5695.

Creative Enzymes is the only company that could provide complete assay services for blood-group-substance endo-1,4-beta-galactosidase. We are a group of outstanding scientists who have been working on enzyme activity assays for many years. Creative Enzymes, equipped with the superb technology, professional knowledge, and the most advanced instruments, support the customers’ research in an excellent way. We will always provide the assay services of high satisfaction and utmost quality.