Creative Enzymes provides activity measurement services for a variety of enzymes. We have been a leading company in providing accurate and reproducible spectrophotometric activity assays. The cutting-edge instruments and extensive experiences allow us to deliver testing results with high accuracy and efficiency. A high standard of operation and execution is maintained through all steps during the measurement. Here, we are proud to provide the activity assay for 6-phospho-beta-galactosidase.

6-Phospho-beta-galactosidase (PGALase, EC 3.2.1.85) was first discovered in Staphylococcus aureus and the same catalytic activity was later demonstrated in Lactococci and Lactobacilli. According to the CAZy database, PGALase is assigned to GH family 1, which comprises glycosidases from organisms as far apart as archaea and man. PAGLases are enzymes that hydrolyze the beta-glycosidic bond between a terminal nonreducing galactose-6-phosphate and other organic molecules.

PGALase participates in vital metabolism activities. This enzyme is usually associated with the phosphotransferase system, which is also referred to as phosphoenolpyruvate-carbohydrate phosphotransferase system (PEP-PTS). This unique transport system is only found in bacteria. PEP-PTS is an alternative intake system involved in the transport and phosphorylation of numerous monosaccharides, disaccharides, amino sugars, polyols, and a series of other sugar derivatives. PGALase is involved in the utilization of lactose. Lactose is transported into the cell, phosphorylated via PEP-PTS system, and further hydrolyzed inside the cell by PGALase, resulting in glucose and galactose-6-phosphate. Up till now, only a small number of these enzymes have been biochemically characterized, among them, only PGALase from L. lactis has been subjected to full crystallographic analysis. Therefore, more and more studies are focused on the structural and the functional exploration in PEP-PTS. To meet different research demands, Creative Enzymes provides the activity assay service for 6-phospho-beta-galactosidase with high quality and reproducibility.

Figure: The three-dimensional structure of 6-phospho-beta galactosidase from Lactococcus lactis.
Reference: Wiesmann, C. et al. Structure 1995 3: 961-968.

Creative Enzymes has established a premier benchmark in activity assays for 6-phospho-beta-galactosidase. We have been specialized in enzyme activity measurement for many years. Based on the cutting-edge equipment and extensive experiences, Creative Enzymes maintains a leading position in this field. The highly reliable results for every request are endorsed by thousands of customers. In the future, Creative Enzymes will continue the support to your research and stay as the best choice for enzyme activity determination.